Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28002 | 3' | -54.2 | NC_005887.1 | + | 26966 | 0.66 | 0.730631 |
Target: 5'- uGCAGGccGUUCGGcGACGGUGUGcCggUGa -3' miRNA: 3'- -CGUCC--UAGGCC-UUGCCGCACaGaaGC- -5' |
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28002 | 3' | -54.2 | NC_005887.1 | + | 5187 | 0.66 | 0.698048 |
Target: 5'- cGCAGGAgaaCGuGaAGCGGCugaucGUCUUCGg -3' miRNA: 3'- -CGUCCUag-GC-C-UUGCCGca---CAGAAGC- -5' |
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28002 | 3' | -54.2 | NC_005887.1 | + | 7575 | 0.67 | 0.674868 |
Target: 5'- gGCAGGcguAUCCGGAcggcaucgcguaaGCGGCGccgGUUgaUCGa -3' miRNA: 3'- -CGUCC---UAGGCCU-------------UGCCGCa--CAGa-AGC- -5' |
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28002 | 3' | -54.2 | NC_005887.1 | + | 37290 | 0.67 | 0.674868 |
Target: 5'- uGCGGGcugaucuUCCGGcGCGGCcauugccGUGcCUUCGu -3' miRNA: 3'- -CGUCCu------AGGCCuUGCCG-------CACaGAAGC- -5' |
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28002 | 3' | -54.2 | NC_005887.1 | + | 26115 | 0.67 | 0.664871 |
Target: 5'- aGCAGGAugUCCuGcACGGCGcG-CUUCGu -3' miRNA: 3'- -CGUCCU--AGGcCuUGCCGCaCaGAAGC- -5' |
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28002 | 3' | -54.2 | NC_005887.1 | + | 38199 | 0.67 | 0.642576 |
Target: 5'- -gAGGAUCUGGAACaGCGUGa----- -3' miRNA: 3'- cgUCCUAGGCCUUGcCGCACagaagc -5' |
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28002 | 3' | -54.2 | NC_005887.1 | + | 34435 | 0.68 | 0.620242 |
Target: 5'- uCGGuuUCCGGAGCGGUcgccucgaaGUcGUCUUCGu -3' miRNA: 3'- cGUCcuAGGCCUUGCCG---------CA-CAGAAGC- -5' |
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28002 | 3' | -54.2 | NC_005887.1 | + | 41939 | 0.7 | 0.489617 |
Target: 5'- aGCGGG---CGGcGCGGCGcGUCUUCGc -3' miRNA: 3'- -CGUCCuagGCCuUGCCGCaCAGAAGC- -5' |
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28002 | 3' | -54.2 | NC_005887.1 | + | 33602 | 0.73 | 0.314616 |
Target: 5'- uGCAGGuUCUGcgcGAgcGCGGCGUgGUCUUCGa -3' miRNA: 3'- -CGUCCuAGGC---CU--UGCCGCA-CAGAAGC- -5' |
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28002 | 3' | -54.2 | NC_005887.1 | + | 16483 | 0.74 | 0.299191 |
Target: 5'- gGCAGGAUCCGGcgggcugguCGGCGaUGUCg--- -3' miRNA: 3'- -CGUCCUAGGCCuu-------GCCGC-ACAGaagc -5' |
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28002 | 3' | -54.2 | NC_005887.1 | + | 39968 | 1.11 | 0.000795 |
Target: 5'- cGCAGGAUCCGGAACGGCGUGUCUUCGu -3' miRNA: 3'- -CGUCCUAGGCCUUGCCGCACAGAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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