Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28004 | 3' | -56.4 | NC_005887.1 | + | 40016 | 0.66 | 0.527792 |
Target: 5'- -cGCGGUGcgcaucauGGCGcgGCUCgaggcgGCCGGCc -3' miRNA: 3'- aaCGCCAU--------CUGCuaCGGGaa----CGGCUG- -5' |
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28004 | 3' | -56.4 | NC_005887.1 | + | 40384 | 0.67 | 0.495488 |
Target: 5'- -cGCGcGaGGAaaucgUGAUGCCCggcacgGCCGACg -3' miRNA: 3'- aaCGC-CaUCU-----GCUACGGGaa----CGGCUG- -5' |
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28004 | 3' | -56.4 | NC_005887.1 | + | 26098 | 0.67 | 0.491248 |
Target: 5'- -aGCucGgcGACGGUGCCCgugcgcucgccgGCCGGCg -3' miRNA: 3'- aaCGc-CauCUGCUACGGGaa----------CGGCUG- -5' |
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28004 | 3' | -56.4 | NC_005887.1 | + | 17477 | 0.67 | 0.481769 |
Target: 5'- -cGCGGcaucggccgccgccUGGACGGUGaaugaCCUgaucGCCGACu -3' miRNA: 3'- aaCGCC--------------AUCUGCUACg----GGAa---CGGCUG- -5' |
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28004 | 3' | -56.4 | NC_005887.1 | + | 37434 | 0.67 | 0.453883 |
Target: 5'- gUGCGG-AGugGAgcgcgGCgCUUGCCuGCg -3' miRNA: 3'- aACGCCaUCugCUa----CGgGAACGGcUG- -5' |
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28004 | 3' | -56.4 | NC_005887.1 | + | 2410 | 0.69 | 0.376814 |
Target: 5'- -cGCuGGcuucgAGGCGAaGCCCgcGCCGACg -3' miRNA: 3'- aaCG-CCa----UCUGCUaCGGGaaCGGCUG- -5' |
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28004 | 3' | -56.4 | NC_005887.1 | + | 13291 | 0.69 | 0.358971 |
Target: 5'- aUGCGGUugAGaACGAUGUaCCg-GCCGGCg -3' miRNA: 3'- aACGCCA--UC-UGCUACG-GGaaCGGCUG- -5' |
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28004 | 3' | -56.4 | NC_005887.1 | + | 14126 | 0.7 | 0.333333 |
Target: 5'- -aGCGGgcagaaccAGAuCGAugUGCCCgaGCCGACg -3' miRNA: 3'- aaCGCCa-------UCU-GCU--ACGGGaaCGGCUG- -5' |
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28004 | 3' | -56.4 | NC_005887.1 | + | 35829 | 0.7 | 0.309063 |
Target: 5'- -gGCGG-GGGCGAUGCCgucaUUGCCGu- -3' miRNA: 3'- aaCGCCaUCUGCUACGGg---AACGGCug -5' |
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28004 | 3' | -56.4 | NC_005887.1 | + | 28830 | 0.71 | 0.286164 |
Target: 5'- -cGCGaucGUGGcGCGcAUGCCCgUGCCGGCg -3' miRNA: 3'- aaCGC---CAUC-UGC-UACGGGaACGGCUG- -5' |
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28004 | 3' | -56.4 | NC_005887.1 | + | 1085 | 0.71 | 0.278834 |
Target: 5'- -cGCaGGUcGACGgcGCCaUUGCCGACg -3' miRNA: 3'- aaCG-CCAuCUGCuaCGGgAACGGCUG- -5' |
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28004 | 3' | -56.4 | NC_005887.1 | + | 30935 | 0.72 | 0.231671 |
Target: 5'- gUUGCGcucAGACGAUGCCUUggcggcGCCGGCc -3' miRNA: 3'- -AACGCca-UCUGCUACGGGAa-----CGGCUG- -5' |
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28004 | 3' | -56.4 | NC_005887.1 | + | 5013 | 0.72 | 0.219488 |
Target: 5'- aUGCGGc-GAcCGAUGCCagUGCCGGCg -3' miRNA: 3'- aACGCCauCU-GCUACGGgaACGGCUG- -5' |
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28004 | 3' | -56.4 | NC_005887.1 | + | 21044 | 0.74 | 0.18109 |
Target: 5'- -cGCGGUGGGCG-UGCCCgugaaUGUgGGCg -3' miRNA: 3'- aaCGCCAUCUGCuACGGGa----ACGgCUG- -5' |
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28004 | 3' | -56.4 | NC_005887.1 | + | 38844 | 1.08 | 0.000542 |
Target: 5'- gUUGCGGUAGACGAUGCCCUUGCCGACg -3' miRNA: 3'- -AACGCCAUCUGCUACGGGAACGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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