Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28005 | 5' | -58.5 | NC_005887.1 | + | 34686 | 0.66 | 0.483003 |
Target: 5'- gUUGuCGAGCAGCaGCGGG-CGGCc-- -3' miRNA: 3'- aAGC-GCUCGUUGcCGCCCaGCUGcuc -5' |
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28005 | 5' | -58.5 | NC_005887.1 | + | 13664 | 0.66 | 0.481985 |
Target: 5'- uUUCGCGGGCGugAUcGCGGGUugucgucUGGCGAa -3' miRNA: 3'- -AAGCGCUCGU--UGcCGCCCA-------GCUGCUc -5' |
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28005 | 5' | -58.5 | NC_005887.1 | + | 39430 | 0.66 | 0.472874 |
Target: 5'- cUUGCGcAGCuugGCGGCGGcGUCGuucGCGGc -3' miRNA: 3'- aAGCGC-UCGu--UGCCGCC-CAGC---UGCUc -5' |
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28005 | 5' | -58.5 | NC_005887.1 | + | 22721 | 0.66 | 0.466848 |
Target: 5'- -cCGUGAGCGAgauCGGCGGGacacugcucgcgaagUCgGACGAc -3' miRNA: 3'- aaGCGCUCGUU---GCCGCCC---------------AG-CUGCUc -5' |
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28005 | 5' | -58.5 | NC_005887.1 | + | 9077 | 0.66 | 0.461857 |
Target: 5'- -gCGCcGGCuGCGGCGGGaCGaaguuucGCGAGa -3' miRNA: 3'- aaGCGcUCGuUGCCGCCCaGC-------UGCUC- -5' |
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28005 | 5' | -58.5 | NC_005887.1 | + | 24941 | 0.66 | 0.459868 |
Target: 5'- aUCGCGAcGCGuGCGcGCGGGcCGccuugcgcgcggccGCGAGg -3' miRNA: 3'- aAGCGCU-CGU-UGC-CGCCCaGC--------------UGCUC- -5' |
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28005 | 5' | -58.5 | NC_005887.1 | + | 41928 | 0.66 | 0.443153 |
Target: 5'- --aGCGcAGCAACaGCGGG-CGGCGcGg -3' miRNA: 3'- aagCGC-UCGUUGcCGCCCaGCUGCuC- -5' |
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28005 | 5' | -58.5 | NC_005887.1 | + | 19811 | 0.67 | 0.433481 |
Target: 5'- -gCGCGAccggaAACGGgGGcGUCGACGAc -3' miRNA: 3'- aaGCGCUcg---UUGCCgCC-CAGCUGCUc -5' |
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28005 | 5' | -58.5 | NC_005887.1 | + | 10221 | 0.67 | 0.414513 |
Target: 5'- gUUGCGAGCAGCGcu--GUCGACGAc -3' miRNA: 3'- aAGCGCUCGUUGCcgccCAGCUGCUc -5' |
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28005 | 5' | -58.5 | NC_005887.1 | + | 34935 | 0.67 | 0.405222 |
Target: 5'- -aCGa-GGCGGCGGCGGGUgCGACc-- -3' miRNA: 3'- aaGCgcUCGUUGCCGCCCA-GCUGcuc -5' |
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28005 | 5' | -58.5 | NC_005887.1 | + | 13384 | 0.68 | 0.378152 |
Target: 5'- cUCGCGcugaaggccGGC-GCGGCGGacGUCGGCGGc -3' miRNA: 3'- aAGCGC---------UCGuUGCCGCC--CAGCUGCUc -5' |
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28005 | 5' | -58.5 | NC_005887.1 | + | 37888 | 0.68 | 0.378152 |
Target: 5'- cUUCGCG-GCAGC--CGGGUCGaacuGCGAGa -3' miRNA: 3'- -AAGCGCuCGUUGccGCCCAGC----UGCUC- -5' |
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28005 | 5' | -58.5 | NC_005887.1 | + | 28572 | 0.68 | 0.360792 |
Target: 5'- gUCGCcgcccGCugcGCGGaCGcGGUCGACGAGg -3' miRNA: 3'- aAGCGcu---CGu--UGCC-GC-CCAGCUGCUC- -5' |
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28005 | 5' | -58.5 | NC_005887.1 | + | 13912 | 0.68 | 0.360792 |
Target: 5'- aUUCGUG-GC-ACGuacGCGGuGUCGACGGGg -3' miRNA: 3'- -AAGCGCuCGuUGC---CGCC-CAGCUGCUC- -5' |
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28005 | 5' | -58.5 | NC_005887.1 | + | 18914 | 0.68 | 0.360792 |
Target: 5'- --aGCGGcGgGAUGGCGGGcggcugCGACGAGc -3' miRNA: 3'- aagCGCU-CgUUGCCGCCCa-----GCUGCUC- -5' |
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28005 | 5' | -58.5 | NC_005887.1 | + | 15619 | 0.68 | 0.352324 |
Target: 5'- gUCGCGcAGCAgGCGGcCGGGcUCGcCGAu -3' miRNA: 3'- aAGCGC-UCGU-UGCC-GCCC-AGCuGCUc -5' |
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28005 | 5' | -58.5 | NC_005887.1 | + | 12648 | 0.68 | 0.352324 |
Target: 5'- -cCGCaGGUuACGaGUGGGUCGGCGAu -3' miRNA: 3'- aaGCGcUCGuUGC-CGCCCAGCUGCUc -5' |
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28005 | 5' | -58.5 | NC_005887.1 | + | 12144 | 0.68 | 0.352324 |
Target: 5'- -cCGCGAcGCGGCguGGCuGGcCGGCGAGa -3' miRNA: 3'- aaGCGCU-CGUUG--CCGcCCaGCUGCUC- -5' |
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28005 | 5' | -58.5 | NC_005887.1 | + | 33470 | 0.68 | 0.343996 |
Target: 5'- -cCGCGAGCGcguACGGCGucgcgaucUCGACGGGc -3' miRNA: 3'- aaGCGCUCGU---UGCCGCcc------AGCUGCUC- -5' |
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28005 | 5' | -58.5 | NC_005887.1 | + | 38640 | 0.68 | 0.335812 |
Target: 5'- aUCGCGGuCGGCGcGCGGcUCGACGGc -3' miRNA: 3'- aAGCGCUcGUUGC-CGCCcAGCUGCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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