Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28006 | 5' | -56.6 | NC_005887.1 | + | 40105 | 0.66 | 0.53678 |
Target: 5'- uGCUGUGGGACGA-GCCGcaggacgccuacAGCgUGCg- -3' miRNA: 3'- uUGACGCUCUGCUaCGGC------------UCG-ACGac -5' |
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28006 | 5' | -56.6 | NC_005887.1 | + | 32629 | 0.66 | 0.53678 |
Target: 5'- cAGCUGCcGGGC---GuuGAGCUGCUGc -3' miRNA: 3'- -UUGACGcUCUGcuaCggCUCGACGAC- -5' |
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28006 | 5' | -56.6 | NC_005887.1 | + | 356 | 0.66 | 0.53678 |
Target: 5'- cGAC-GUGGGcCGcagGCCGuGCUGCUGa -3' miRNA: 3'- -UUGaCGCUCuGCua-CGGCuCGACGAC- -5' |
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28006 | 5' | -56.6 | NC_005887.1 | + | 38252 | 0.66 | 0.51499 |
Target: 5'- -cCUGCGcGAacuCGAUGCCGAuCUGCg- -3' miRNA: 3'- uuGACGCuCU---GCUACGGCUcGACGac -5' |
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28006 | 5' | -56.6 | NC_005887.1 | + | 7655 | 0.66 | 0.504229 |
Target: 5'- ---aGCGcGACGAcgGCCGcccGCUGCUGc -3' miRNA: 3'- uugaCGCuCUGCUa-CGGCu--CGACGAC- -5' |
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28006 | 5' | -56.6 | NC_005887.1 | + | 17628 | 0.66 | 0.493566 |
Target: 5'- cGCUGCGcacGACGGUcagcGCCGugaAGCUGCa- -3' miRNA: 3'- uUGACGCu--CUGCUA----CGGC---UCGACGac -5' |
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28006 | 5' | -56.6 | NC_005887.1 | + | 1418 | 0.67 | 0.472561 |
Target: 5'- cAGCgGCaAGACGAacgCGAGCUGCUGg -3' miRNA: 3'- -UUGaCGcUCUGCUacgGCUCGACGAC- -5' |
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28006 | 5' | -56.6 | NC_005887.1 | + | 17429 | 0.67 | 0.452015 |
Target: 5'- ---cGCGAucgacaACGGUGCCGAuccgGCUGCUGc -3' miRNA: 3'- uugaCGCUc-----UGCUACGGCU----CGACGAC- -5' |
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28006 | 5' | -56.6 | NC_005887.1 | + | 14985 | 0.67 | 0.452015 |
Target: 5'- ----uCGAGACGAUGCCGAcGCUcgucugcgcgccGCUGc -3' miRNA: 3'- uugacGCUCUGCUACGGCU-CGA------------CGAC- -5' |
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28006 | 5' | -56.6 | NC_005887.1 | + | 5014 | 0.67 | 0.441927 |
Target: 5'- uGCgGCGAccGAUGccaGUGCCGGcGCUGCUGc -3' miRNA: 3'- uUGaCGCU--CUGC---UACGGCU-CGACGAC- -5' |
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28006 | 5' | -56.6 | NC_005887.1 | + | 21161 | 0.68 | 0.384218 |
Target: 5'- cGACgGCGuuuuCGAUGCCGAGUUGUUc -3' miRNA: 3'- -UUGaCGCucu-GCUACGGCUCGACGAc -5' |
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28006 | 5' | -56.6 | NC_005887.1 | + | 29187 | 0.68 | 0.384218 |
Target: 5'- cGCUGCGAGgaucuGCGcgGCCuuaAGCUGCg- -3' miRNA: 3'- uUGACGCUC-----UGCuaCGGc--UCGACGac -5' |
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28006 | 5' | -56.6 | NC_005887.1 | + | 7024 | 0.69 | 0.357297 |
Target: 5'- -cCUGgGAGACGGUGUCGGGUgaacGCg- -3' miRNA: 3'- uuGACgCUCUGCUACGGCUCGa---CGac -5' |
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28006 | 5' | -56.6 | NC_005887.1 | + | 30809 | 0.69 | 0.348623 |
Target: 5'- ---cGCGcuGCGc-GCCGAGCUGCUGa -3' miRNA: 3'- uugaCGCucUGCuaCGGCUCGACGAC- -5' |
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28006 | 5' | -56.6 | NC_005887.1 | + | 11431 | 0.69 | 0.348623 |
Target: 5'- cGCUGUGcacaAGGCGAUGCCGcGCUGg-- -3' miRNA: 3'- uUGACGC----UCUGCUACGGCuCGACgac -5' |
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28006 | 5' | -56.6 | NC_005887.1 | + | 23430 | 0.69 | 0.340099 |
Target: 5'- cGCUGCGAGAuuCGGUGagcgcugCGGGCUGCc- -3' miRNA: 3'- uUGACGCUCU--GCUACg------GCUCGACGac -5' |
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28006 | 5' | -56.6 | NC_005887.1 | + | 2535 | 0.69 | 0.323509 |
Target: 5'- cGCUGCGcaAGGCGcUGUCGGGCgGCUa -3' miRNA: 3'- uUGACGC--UCUGCuACGGCUCGaCGAc -5' |
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28006 | 5' | -56.6 | NC_005887.1 | + | 26460 | 0.74 | 0.165547 |
Target: 5'- cAGCUGCGcGuCGAgcgcaCCGAGCUGCUGg -3' miRNA: 3'- -UUGACGCuCuGCUac---GGCUCGACGAC- -5' |
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28006 | 5' | -56.6 | NC_005887.1 | + | 37906 | 0.96 | 0.003616 |
Target: 5'- gAACUGCGAGACGAUGCCG-GCUGCUGc -3' miRNA: 3'- -UUGACGCUCUGCUACGGCuCGACGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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