Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28007 | 3' | -59.8 | NC_005887.1 | + | 41201 | 0.68 | 0.325255 |
Target: 5'- gGCCCGUCAGAUCCUucacgCGCaCG-C-GCGg -3' miRNA: 3'- gCGGGUAGUCUGGGA-----GCG-GCaGaCGC- -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 41191 | 0.66 | 0.438434 |
Target: 5'- cCGCCuggcugCAUCcGACCCgcgCGUCGUCgUGCc -3' miRNA: 3'- -GCGG------GUAGuCUGGGa--GCGGCAG-ACGc -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 40741 | 0.66 | 0.40123 |
Target: 5'- cCGCCCgcGUCgAGGCgaUCGCCGUUgGCGc -3' miRNA: 3'- -GCGGG--UAG-UCUGggAGCGGCAGaCGC- -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 37668 | 1.08 | 0.000354 |
Target: 5'- gCGCCCAUCAGACCCUCGCCGUCUGCGc -3' miRNA: 3'- -GCGGGUAGUCUGGGAGCGGCAGACGC- -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 36525 | 0.66 | 0.428947 |
Target: 5'- uCGCCCuUCGcGACCCgCGCCGaCU-CGa -3' miRNA: 3'- -GCGGGuAGU-CUGGGaGCGGCaGAcGC- -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 35404 | 0.66 | 0.426125 |
Target: 5'- gCGCCCGcgCAcGGCCUUCGCCcacgagucgaucagGUCgaGCGa -3' miRNA: 3'- -GCGGGUa-GU-CUGGGAGCGG--------------CAGa-CGC- -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 34619 | 0.68 | 0.333146 |
Target: 5'- uGCCCGcCAG-CCaUUCGCCGUUcGCGu -3' miRNA: 3'- gCGGGUaGUCuGG-GAGCGGCAGaCGC- -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 34519 | 0.76 | 0.089694 |
Target: 5'- aCGCCCAUCAGGCCgCagcggaugcccggguUCGUcuuCGUCUGCGc -3' miRNA: 3'- -GCGGGUAGUCUGG-G---------------AGCG---GCAGACGC- -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 34446 | 0.66 | 0.40123 |
Target: 5'- aGUCCGUuacugCAGAUCgUCGCCGgcCUGCu -3' miRNA: 3'- gCGGGUA-----GUCUGGgAGCGGCa-GACGc -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 30423 | 0.7 | 0.241383 |
Target: 5'- uCGCCCAccaUCAGGCCgaCGUCGgg-GCGg -3' miRNA: 3'- -GCGGGU---AGUCUGGgaGCGGCagaCGC- -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 30149 | 0.73 | 0.150031 |
Target: 5'- gCGCCCGgcgCGGcgcuguCCUUCGCgGUCUGCa -3' miRNA: 3'- -GCGGGUa--GUCu-----GGGAGCGgCAGACGc -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 29171 | 0.66 | 0.410343 |
Target: 5'- gGCCUGcUC-GACCgCUccCGCUGUCUGCGc -3' miRNA: 3'- gCGGGU-AGuCUGG-GA--GCGGCAGACGC- -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 27079 | 0.69 | 0.267148 |
Target: 5'- uCGCCgG-CGGACgCUCGCCGcgcagcaCUGCGg -3' miRNA: 3'- -GCGGgUaGUCUGgGAGCGGCa------GACGC- -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 26719 | 0.67 | 0.349346 |
Target: 5'- gCGCCCugAUCGGcgaGCCCg-GCCGcCUGCu -3' miRNA: 3'- -GCGGG--UAGUC---UGGGagCGGCaGACGc -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 25875 | 0.66 | 0.392248 |
Target: 5'- aGCCCGcCGGAUCCUgccgCGCgGUCgaGCGc -3' miRNA: 3'- gCGGGUaGUCUGGGA----GCGgCAGa-CGC- -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 25433 | 0.69 | 0.287897 |
Target: 5'- gCGCCCGUCG---UCUUGCCGaUCUGCu -3' miRNA: 3'- -GCGGGUAGUcugGGAGCGGC-AGACGc -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 24974 | 0.66 | 0.438434 |
Target: 5'- gCGUUCAaCAGcgaGCCCUCGCCGaacugGCGg -3' miRNA: 3'- -GCGGGUaGUC---UGGGAGCGGCaga--CGC- -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 24101 | 0.66 | 0.40123 |
Target: 5'- --aUCAUguGGCCCUUgGCCGUgCUGCGc -3' miRNA: 3'- gcgGGUAguCUGGGAG-CGGCA-GACGC- -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 22876 | 0.67 | 0.357654 |
Target: 5'- gGCCgcUAUCAGAUCCUCGCgCG-CUGg- -3' miRNA: 3'- gCGG--GUAGUCUGGGAGCG-GCaGACgc -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 20701 | 0.66 | 0.438434 |
Target: 5'- aCGCCCGUCAGAUUggUCGgCGUCggGUa -3' miRNA: 3'- -GCGGGUAGUCUGGg-AGCgGCAGa-CGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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