miRNA display CGI


Results 21 - 25 of 25 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
28007 3' -59.8 NC_005887.1 + 5271 0.7 0.247624
Target:  5'- uCGCCCGcuUCGGugCgaaCGCCGUCaGCGa -3'
miRNA:   3'- -GCGGGU--AGUCugGga-GCGGCAGaCGC- -5'
28007 3' -59.8 NC_005887.1 + 30149 0.73 0.150031
Target:  5'- gCGCCCGgcgCGGcgcuguCCUUCGCgGUCUGCa -3'
miRNA:   3'- -GCGGGUa--GUCu-----GGGAGCGgCAGACGc -5'
28007 3' -59.8 NC_005887.1 + 1717 0.73 0.154152
Target:  5'- gCGCUCGUCAGugCCUgucuUGCCGaUgUGCGg -3'
miRNA:   3'- -GCGGGUAGUCugGGA----GCGGC-AgACGC- -5'
28007 3' -59.8 NC_005887.1 + 34519 0.76 0.089694
Target:  5'- aCGCCCAUCAGGCCgCagcggaugcccggguUCGUcuuCGUCUGCGc -3'
miRNA:   3'- -GCGGGUAGUCUGG-G---------------AGCG---GCAGACGC- -5'
28007 3' -59.8 NC_005887.1 + 37668 1.08 0.000354
Target:  5'- gCGCCCAUCAGACCCUCGCCGUCUGCGc -3'
miRNA:   3'- -GCGGGUAGUCUGGGAGCGGCAGACGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.