Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28007 | 3' | -59.8 | NC_005887.1 | + | 5271 | 0.7 | 0.247624 |
Target: 5'- uCGCCCGcuUCGGugCgaaCGCCGUCaGCGa -3' miRNA: 3'- -GCGGGU--AGUCugGga-GCGGCAGaCGC- -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 30149 | 0.73 | 0.150031 |
Target: 5'- gCGCCCGgcgCGGcgcuguCCUUCGCgGUCUGCa -3' miRNA: 3'- -GCGGGUa--GUCu-----GGGAGCGgCAGACGc -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 1717 | 0.73 | 0.154152 |
Target: 5'- gCGCUCGUCAGugCCUgucuUGCCGaUgUGCGg -3' miRNA: 3'- -GCGGGUAGUCugGGA----GCGGC-AgACGC- -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 34519 | 0.76 | 0.089694 |
Target: 5'- aCGCCCAUCAGGCCgCagcggaugcccggguUCGUcuuCGUCUGCGc -3' miRNA: 3'- -GCGGGUAGUCUGG-G---------------AGCG---GCAGACGC- -5' |
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28007 | 3' | -59.8 | NC_005887.1 | + | 37668 | 1.08 | 0.000354 |
Target: 5'- gCGCCCAUCAGACCCUCGCCGUCUGCGc -3' miRNA: 3'- -GCGGGUAGUCUGGGAGCGGCAGACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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