Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28008 | 5' | -56.3 | NC_005887.1 | + | 30367 | 0.66 | 0.634392 |
Target: 5'- -uGCCGAGCUCGGUGUaCCGcUCGg- -3' miRNA: 3'- gcUGGCUCGAGCUGCAaGGCcAGCau -5' |
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28008 | 5' | -56.3 | NC_005887.1 | + | 24744 | 0.66 | 0.616763 |
Target: 5'- gCGaACCGAGCUucgggccggCGACGUuguccagaucccacgUCCGGUaGUAg -3' miRNA: 3'- -GC-UGGCUCGA---------GCUGCA---------------AGGCCAgCAU- -5' |
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28008 | 5' | -56.3 | NC_005887.1 | + | 41229 | 0.66 | 0.60906 |
Target: 5'- uGACCGAGCUCGACaagcacacgcaccuGcUCgGcGUCGg- -3' miRNA: 3'- gCUGGCUCGAGCUG--------------CaAGgC-CAGCau -5' |
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28008 | 5' | -56.3 | NC_005887.1 | + | 33919 | 0.66 | 0.590398 |
Target: 5'- uGACgCGGaucGCUCGGCGauagcCUGGUCGUGc -3' miRNA: 3'- gCUG-GCU---CGAGCUGCaa---GGCCAGCAU- -5' |
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28008 | 5' | -56.3 | NC_005887.1 | + | 1974 | 0.66 | 0.579465 |
Target: 5'- gCGGCCGAGaacaUCGACaaccUCCGGcCGg- -3' miRNA: 3'- -GCUGGCUCg---AGCUGca--AGGCCaGCau -5' |
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28008 | 5' | -56.3 | NC_005887.1 | + | 2002 | 0.67 | 0.557738 |
Target: 5'- aCGGCCGcGCUCGACGgccaguccaUUUCGG-CGg- -3' miRNA: 3'- -GCUGGCuCGAGCUGC---------AAGGCCaGCau -5' |
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28008 | 5' | -56.3 | NC_005887.1 | + | 16473 | 0.67 | 0.54696 |
Target: 5'- uCGACCGcGCggcaggaucCGGCGggCUGGUCGg- -3' miRNA: 3'- -GCUGGCuCGa--------GCUGCaaGGCCAGCau -5' |
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28008 | 5' | -56.3 | NC_005887.1 | + | 15631 | 0.67 | 0.536249 |
Target: 5'- gCGGCCGGGCUCGcCGaucagggcgCCGuGUCGc- -3' miRNA: 3'- -GCUGGCUCGAGCuGCaa-------GGC-CAGCau -5' |
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28008 | 5' | -56.3 | NC_005887.1 | + | 26737 | 0.67 | 0.536249 |
Target: 5'- aGACCGGGCgcaCGAuCGgcucgUCCaGGUCGa- -3' miRNA: 3'- gCUGGCUCGa--GCU-GCa----AGG-CCAGCau -5' |
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28008 | 5' | -56.3 | NC_005887.1 | + | 11475 | 0.68 | 0.494211 |
Target: 5'- uGACCGAGCUCG-CGcUCUGG-Ca-- -3' miRNA: 3'- gCUGGCUCGAGCuGCaAGGCCaGcau -5' |
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28008 | 5' | -56.3 | NC_005887.1 | + | 31301 | 0.68 | 0.463693 |
Target: 5'- uCGACCG-GCaUCGGCGcaCCGGUgGUc -3' miRNA: 3'- -GCUGGCuCG-AGCUGCaaGGCCAgCAu -5' |
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28008 | 5' | -56.3 | NC_005887.1 | + | 40405 | 0.69 | 0.434191 |
Target: 5'- cCGGCaCG-GC-CGACGUgCCGGUCGa- -3' miRNA: 3'- -GCUG-GCuCGaGCUGCAaGGCCAGCau -5' |
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28008 | 5' | -56.3 | NC_005887.1 | + | 14690 | 0.69 | 0.415138 |
Target: 5'- aCGAUCGGGCUCGucgcggcggGCGggCCGGgcgCGg- -3' miRNA: 3'- -GCUGGCUCGAGC---------UGCaaGGCCa--GCau -5' |
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28008 | 5' | -56.3 | NC_005887.1 | + | 39864 | 0.7 | 0.361184 |
Target: 5'- aGGCCGGGCUCGcCGUcgaugaUCCGcGUCa-- -3' miRNA: 3'- gCUGGCUCGAGCuGCA------AGGC-CAGcau -5' |
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28008 | 5' | -56.3 | NC_005887.1 | + | 17750 | 0.71 | 0.356064 |
Target: 5'- aCGGCCGcgcGGCUgaUGGCGcccgguucgaaggacUUCCGGUCGUGg -3' miRNA: 3'- -GCUGGC---UCGA--GCUGC---------------AAGGCCAGCAU- -5' |
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28008 | 5' | -56.3 | NC_005887.1 | + | 36458 | 1.07 | 0.000936 |
Target: 5'- gCGACCGAGCUCGACGUUCCGGUCGUAg -3' miRNA: 3'- -GCUGGCUCGAGCUGCAAGGCCAGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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