Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2801 | 3' | -58.9 | NC_001491.2 | + | 85696 | 0.66 | 0.831844 |
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Target: 5'- gGGuGUCUCCUCCggccacggcggCGUgGCCcaCGUCc -3' miRNA: 3'- -CC-CAGAGGAGGa----------GCGgCGGaaGCAGc -5' |
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| 2801 | 3' | -58.9 | NC_001491.2 | + | 145685 | 0.66 | 0.806904 |
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Target: 5'- cGGGg--CCUCCUCgGCCgguGCCUggGUCu -3' miRNA: 3'- -CCCagaGGAGGAG-CGG---CGGAagCAGc -5' |
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| 2801 | 3' | -58.9 | NC_001491.2 | + | 60679 | 0.67 | 0.780631 |
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Target: 5'- aGGcGUCcCCcaaCUCGCCgGCCccgUCGUCGg -3' miRNA: 3'- -CC-CAGaGGag-GAGCGG-CGGa--AGCAGC- -5' |
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| 2801 | 3' | -58.9 | NC_001491.2 | + | 58881 | 0.68 | 0.724944 |
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Target: 5'- aGGGcaUCCagaCUCGCCGCCaaaaacUCGUCGu -3' miRNA: 3'- -CCCagAGGag-GAGCGGCGGa-----AGCAGC- -5' |
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| 2801 | 3' | -58.9 | NC_001491.2 | + | 118015 | 0.68 | 0.724944 |
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Target: 5'- cGGGgcgaguagCUCCUCUUCGUCGuCCUcCGaCGa -3' miRNA: 3'- -CCCa-------GAGGAGGAGCGGC-GGAaGCaGC- -5' |
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| 2801 | 3' | -58.9 | NC_001491.2 | + | 138139 | 0.71 | 0.558178 |
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Target: 5'- cGG-CgCCUCCUCGCCGCUggaGUUGg -3' miRNA: 3'- cCCaGaGGAGGAGCGGCGGaagCAGC- -5' |
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| 2801 | 3' | -58.9 | NC_001491.2 | + | 89423 | 0.77 | 0.254531 |
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Target: 5'- uGGGUCUCCgaCCUCaCCGCCUccucCGUCGc -3' miRNA: 3'- -CCCAGAGGa-GGAGcGGCGGAa---GCAGC- -5' |
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| 2801 | 3' | -58.9 | NC_001491.2 | + | 15918 | 0.84 | 0.100243 |
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Target: 5'- gGGGUCuUCUUCCUCGUCGUUUUCGUCa -3' miRNA: 3'- -CCCAG-AGGAGGAGCGGCGGAAGCAGc -5' |
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| 2801 | 3' | -58.9 | NC_001491.2 | + | 145252 | 0.97 | 0.011869 |
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Target: 5'- gGGGUCUCCUCCUCGCCGC-UUCGUCGc -3' miRNA: 3'- -CCCAGAGGAGGAGCGGCGgAAGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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