Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2801 | 5' | -55 | NC_001491.2 | + | 145219 | 1.11 | 0.003014 |
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Target: 5'- uGUCGUCGGAGGACGACGAAGAGGAGCu -3' miRNA: 3'- -CAGCAGCCUCCUGCUGCUUCUCCUCG- -5' |
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| 2801 | 5' | -55 | NC_001491.2 | + | 146637 | 0.67 | 0.906264 |
|
Target: 5'- --aGUCcGGGGACGGCGGacgccccgccgcGGAGGcGCa -3' miRNA: 3'- cagCAGcCUCCUGCUGCU------------UCUCCuCG- -5' |
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| 2801 | 5' | -55 | NC_001491.2 | + | 146677 | 0.66 | 0.94784 |
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Target: 5'- cUCG--GGAGGGCaGGCgGGAGAGGGGa -3' miRNA: 3'- cAGCagCCUCCUG-CUG-CUUCUCCUCg -5' |
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| 2801 | 5' | -55 | NC_001491.2 | + | 147276 | 0.67 | 0.906264 |
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Target: 5'- --gGcCGGGGaGGCGGCcgccuGAGGGGAGCg -3' miRNA: 3'- cagCaGCCUC-CUGCUGc----UUCUCCUCG- -5' |
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| 2801 | 5' | -55 | NC_001491.2 | + | 147332 | 0.65 | 0.955904 |
|
Target: 5'- -cCGU-GGAuGGcACGGucgucCGAGGAGGAGCc -3' miRNA: 3'- caGCAgCCU-CC-UGCU-----GCUUCUCCUCG- -5' |
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| 2801 | 5' | -55 | NC_001491.2 | + | 148405 | 0.68 | 0.872747 |
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Target: 5'- -aCGUUGGAGGAgCGGCacAGccucAGGGGCg -3' miRNA: 3'- caGCAGCCUCCU-GCUGcuUC----UCCUCG- -5' |
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| 2801 | 5' | -55 | NC_001491.2 | + | 148794 | 0.71 | 0.762843 |
|
Target: 5'- aGUCGacccgCGGGGcuCGGCGgcGGGGAGCc -3' miRNA: 3'- -CAGCa----GCCUCcuGCUGCuuCUCCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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