Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2801 | 5' | -55 | NC_001491.2 | + | 148794 | 0.71 | 0.762843 |
Target: 5'- aGUCGacccgCGGGGcuCGGCGgcGGGGAGCc -3' miRNA: 3'- -CAGCa----GCCUCcuGCUGCuuCUCCUCG- -5' |
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2801 | 5' | -55 | NC_001491.2 | + | 104589 | 0.71 | 0.75335 |
Target: 5'- uGUCuGUCuGGAGGGCGAuCGAAGAGacGCc -3' miRNA: 3'- -CAG-CAG-CCUCCUGCU-GCUUCUCcuCG- -5' |
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2801 | 5' | -55 | NC_001491.2 | + | 114214 | 0.71 | 0.743751 |
Target: 5'- -gUGUCGGAcGACGAgGAcGGAGGAGa -3' miRNA: 3'- caGCAGCCUcCUGCUgCU-UCUCCUCg -5' |
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2801 | 5' | -55 | NC_001491.2 | + | 13106 | 0.72 | 0.714412 |
Target: 5'- -gCGUUGGAacACGugGggGuGGAGCa -3' miRNA: 3'- caGCAGCCUccUGCugCuuCuCCUCG- -5' |
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2801 | 5' | -55 | NC_001491.2 | + | 101361 | 0.72 | 0.664244 |
Target: 5'- -cCGU-GGAcaagcucauGGGCGAgGGAGAGGAGCu -3' miRNA: 3'- caGCAgCCU---------CCUGCUgCUUCUCCUCG- -5' |
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2801 | 5' | -55 | NC_001491.2 | + | 27257 | 0.79 | 0.317225 |
Target: 5'- uUCGUCGGGGGGCGA-GuGGAGcGAGCu -3' miRNA: 3'- cAGCAGCCUCCUGCUgCuUCUC-CUCG- -5' |
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2801 | 5' | -55 | NC_001491.2 | + | 145219 | 1.11 | 0.003014 |
Target: 5'- uGUCGUCGGAGGACGACGAAGAGGAGCu -3' miRNA: 3'- -CAGCAGCCUCCUGCUGCUUCUCCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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