Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28010 | 5' | -53.3 | NC_005887.1 | + | 34937 | 0.7 | 0.499334 |
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Target: 5'- cUUGCc-GUCGCCGG-CUUCGaacUCGGCGg -3' miRNA: 3'- -AGCGaaCAGCGGCUaGAAGC---AGUCGC- -5' |
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| 28010 | 5' | -53.3 | NC_005887.1 | + | 17386 | 0.71 | 0.447489 |
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Target: 5'- cUCGCUguUGaacgCGCUGGUCgaucaCGUCGGCGg -3' miRNA: 3'- -AGCGA--ACa---GCGGCUAGaa---GCAGUCGC- -5' |
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| 28010 | 5' | -53.3 | NC_005887.1 | + | 29425 | 0.72 | 0.417853 |
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Target: 5'- -aGCUcGUCgGCCGAguagUCGUCGGCGu -3' miRNA: 3'- agCGAaCAG-CGGCUaga-AGCAGUCGC- -5' |
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| 28010 | 5' | -53.3 | NC_005887.1 | + | 35055 | 0.73 | 0.353587 |
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Target: 5'- aCGCgagGUCGCUGccg-UCGUCGGCGg -3' miRNA: 3'- aGCGaa-CAGCGGCuagaAGCAGUCGC- -5' |
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| 28010 | 5' | -53.3 | NC_005887.1 | + | 35019 | 0.74 | 0.312141 |
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Target: 5'- aCGUUUcGUCGCCaGUCgugUUGUCGGCGg -3' miRNA: 3'- aGCGAA-CAGCGGcUAGa--AGCAGUCGC- -5' |
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| 28010 | 5' | -53.3 | NC_005887.1 | + | 34383 | 1.09 | 0.00127 |
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Target: 5'- uUCGCUUGUCGCCGAUCUUCGUCAGCGc -3' miRNA: 3'- -AGCGAACAGCGGCUAGAAGCAGUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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