Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28011 | 3' | -52.1 | NC_005887.1 | + | 37387 | 0.67 | 0.768865 |
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Target: 5'- cUUCUCCuUCAgCUGGC-GCUGcgCGg -3' miRNA: 3'- cAAGAGGuAGUgGACCGuUGGCaaGC- -5' |
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| 28011 | 3' | -52.1 | NC_005887.1 | + | 36442 | 0.74 | 0.343027 |
|
Target: 5'- --aCUUgcgCAUCGCCUGGCGACCGagcUCGa -3' miRNA: 3'- caaGAG---GUAGUGGACCGUUGGCa--AGC- -5' |
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| 28011 | 3' | -52.1 | NC_005887.1 | + | 35989 | 0.69 | 0.6347 |
|
Target: 5'- aGUUgUCCGUUACCUGuGCAGucgagcucUUGUUCGg -3' miRNA: 3'- -CAAgAGGUAGUGGAC-CGUU--------GGCAAGC- -5' |
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| 28011 | 3' | -52.1 | NC_005887.1 | + | 33888 | 0.66 | 0.819538 |
|
Target: 5'- --gCUCCGUCGCCguauUGGUcgUCGUgcUCGg -3' miRNA: 3'- caaGAGGUAGUGG----ACCGuuGGCA--AGC- -5' |
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| 28011 | 3' | -52.1 | NC_005887.1 | + | 33695 | 1.1 | 0.001223 |
|
Target: 5'- aGUUCUCCAUCACCUGGCAACCGUUCGg -3' miRNA: 3'- -CAAGAGGUAGUGGACCGUUGGCAAGC- -5' |
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| 28011 | 3' | -52.1 | NC_005887.1 | + | 30064 | 0.68 | 0.702021 |
|
Target: 5'- --gCUCCAUCACCuugccgagcUGGCGcauguacuugucgACCGcUUCGa -3' miRNA: 3'- caaGAGGUAGUGG---------ACCGU-------------UGGC-AAGC- -5' |
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| 28011 | 3' | -52.1 | NC_005887.1 | + | 27623 | 0.66 | 0.809785 |
|
Target: 5'- uUUCUCgauCGUCGCCUGcGCGAUCGccgCGc -3' miRNA: 3'- cAAGAG---GUAGUGGAC-CGUUGGCaa-GC- -5' |
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| 28011 | 3' | -52.1 | NC_005887.1 | + | 21494 | 0.69 | 0.6347 |
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Target: 5'- -aUCUCgCAUCGCCgccGGCAAUCGgcgCa -3' miRNA: 3'- caAGAG-GUAGUGGa--CCGUUGGCaa-Gc -5' |
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| 28011 | 3' | -52.1 | NC_005887.1 | + | 4933 | 0.67 | 0.758219 |
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Target: 5'- --aCUCCG-CACCUGGCGugCaGgcagUCGc -3' miRNA: 3'- caaGAGGUaGUGGACCGUugG-Ca---AGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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