Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28011 | 5' | -56.6 | NC_005887.1 | + | 257 | 0.67 | 0.49529 |
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Target: 5'- cGUCGACGCGgcaucGCGCCccacGCGACaGCGc -3' miRNA: 3'- -UAGCUGUGCa----CGUGGcu--CGUUGcCGC- -5' |
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| 28011 | 5' | -56.6 | NC_005887.1 | + | 14906 | 0.67 | 0.505794 |
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Target: 5'- cUCGA-ACGUGUggGCCG-GCAugGGCc -3' miRNA: 3'- uAGCUgUGCACG--UGGCuCGUugCCGc -5' |
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| 28011 | 5' | -56.6 | NC_005887.1 | + | 11086 | 0.67 | 0.505794 |
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Target: 5'- gAUCGACGCGgccgacgaGUACUGguGGCAgaACGGUGg -3' miRNA: 3'- -UAGCUGUGCa-------CGUGGC--UCGU--UGCCGC- -5' |
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| 28011 | 5' | -56.6 | NC_005887.1 | + | 26453 | 0.67 | 0.515329 |
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Target: 5'- gGUCGGCcagcugcGCGUcgagcGCACCGAGCuGCuGGUGc -3' miRNA: 3'- -UAGCUG-------UGCA-----CGUGGCUCGuUG-CCGC- -5' |
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| 28011 | 5' | -56.6 | NC_005887.1 | + | 21605 | 0.67 | 0.516393 |
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Target: 5'- -cCGGCGCGUGCAUUGGucacggaaaaauGCugGAUGGCGu -3' miRNA: 3'- uaGCUGUGCACGUGGCU------------CG--UUGCCGC- -5' |
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| 28011 | 5' | -56.6 | NC_005887.1 | + | 13773 | 0.66 | 0.581593 |
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Target: 5'- cGUCGAcCGCGUccgcGCAgCGGGCGGCGaCGc -3' miRNA: 3'- -UAGCU-GUGCA----CGUgGCUCGUUGCcGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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