Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28011 | 5' | -56.6 | NC_005887.1 | + | 15384 | 0.67 | 0.474587 |
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Target: 5'- gGUCGACGCcgcgcugaucGCGCgCGGGCAGCGGa- -3' miRNA: 3'- -UAGCUGUGca--------CGUG-GCUCGUUGCCgc -5' |
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| 28011 | 5' | -56.6 | NC_005887.1 | + | 40763 | 0.68 | 0.464399 |
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Target: 5'- cGUUGGCGCGccGCgcgccGCCGAGC--CGGCGg -3' miRNA: 3'- -UAGCUGUGCa-CG-----UGGCUCGuuGCCGC- -5' |
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| 28011 | 5' | -56.6 | NC_005887.1 | + | 27337 | 0.68 | 0.464399 |
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Target: 5'- cGUCGAC---UGCGCCacgcGCAGCGGCGc -3' miRNA: 3'- -UAGCUGugcACGUGGcu--CGUUGCCGC- -5' |
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| 28011 | 5' | -56.6 | NC_005887.1 | + | 2238 | 0.68 | 0.454327 |
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Target: 5'- gGUCGGaaguCGUcGCGaCGAGCAugGGCGc -3' miRNA: 3'- -UAGCUgu--GCA-CGUgGCUCGUugCCGC- -5' |
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| 28011 | 5' | -56.6 | NC_005887.1 | + | 40231 | 0.68 | 0.444373 |
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Target: 5'- --aGAacaGCGU--GCCGAGCGACGGCu -3' miRNA: 3'- uagCUg--UGCAcgUGGCUCGUUGCCGc -5' |
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| 28011 | 5' | -56.6 | NC_005887.1 | + | 33962 | 0.66 | 0.581593 |
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Target: 5'- -aCGuCGCGUGCGgcguCCGucAGCAcCGGCGc -3' miRNA: 3'- uaGCuGUGCACGU----GGC--UCGUuGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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