Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28013 | 3' | -55.6 | NC_005887.1 | + | 29161 | 0.66 | 0.636821 |
Target: 5'- gUCGGccgGCGGCCUGcucgACCGcucccgcugucUGCGCg -3' miRNA: 3'- -AGCC---UGCCGGACuua-UGGCu----------ACGCGa -5' |
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28013 | 3' | -55.6 | NC_005887.1 | + | 1709 | 0.71 | 0.359459 |
Target: 5'- aUCGGcGCGcgcucgucaguGCCUGucuUGCCGAUGUGCg -3' miRNA: 3'- -AGCC-UGC-----------CGGACuu-AUGGCUACGCGa -5' |
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28013 | 3' | -55.6 | NC_005887.1 | + | 26307 | 0.7 | 0.377112 |
Target: 5'- aUCGG-CGGUCUGcAAUACCGGcuccUGCgGCUc -3' miRNA: 3'- -AGCCuGCCGGAC-UUAUGGCU----ACG-CGA- -5' |
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28013 | 3' | -55.6 | NC_005887.1 | + | 15307 | 0.69 | 0.443311 |
Target: 5'- gUCGGGCGGCa-GcGUGCgCGGUcGCGCg -3' miRNA: 3'- -AGCCUGCCGgaCuUAUG-GCUA-CGCGa -5' |
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28013 | 3' | -55.6 | NC_005887.1 | + | 18926 | 0.69 | 0.463397 |
Target: 5'- gCGGGCGGCUgcgacGAGcugAUCGAcGCGCUg -3' miRNA: 3'- aGCCUGCCGGa----CUUa--UGGCUaCGCGA- -5' |
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28013 | 3' | -55.6 | NC_005887.1 | + | 24377 | 0.67 | 0.558939 |
Target: 5'- gUCGGuGCGGCC--GAUGCCGGcgagcccGCGCa -3' miRNA: 3'- -AGCC-UGCCGGacUUAUGGCUa------CGCGa -5' |
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28013 | 3' | -55.6 | NC_005887.1 | + | 28447 | 0.67 | 0.581018 |
Target: 5'- gCGuACGuGCCacGAAUGCCGAcGCGCa -3' miRNA: 3'- aGCcUGC-CGGa-CUUAUGGCUaCGCGa -5' |
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28013 | 3' | -55.6 | NC_005887.1 | + | 36827 | 0.67 | 0.581018 |
Target: 5'- aUCGuGCGuGCCUGcugcgGCCGGUGCuGCg -3' miRNA: 3'- -AGCcUGC-CGGACuua--UGGCUACG-CGa -5' |
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28013 | 3' | -55.6 | NC_005887.1 | + | 11827 | 0.67 | 0.585457 |
Target: 5'- cUCGGccgacggccgccuacGCGGgCUGAugcaguuUugCGGUGCGCa -3' miRNA: 3'- -AGCC---------------UGCCgGACUu------AugGCUACGCGa -5' |
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28013 | 3' | -55.6 | NC_005887.1 | + | 11027 | 0.66 | 0.614443 |
Target: 5'- cCGGuCGGCacgugggacgUGAccACCGGUGCGCc -3' miRNA: 3'- aGCCuGCCGg---------ACUuaUGGCUACGCGa -5' |
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28013 | 3' | -55.6 | NC_005887.1 | + | 11087 | 0.66 | 0.614443 |
Target: 5'- aUCGacGCGGCCgacGAGUACUGGUG-GCa -3' miRNA: 3'- -AGCc-UGCCGGa--CUUAUGGCUACgCGa -5' |
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28013 | 3' | -55.6 | NC_005887.1 | + | 33216 | 1.08 | 0.000845 |
Target: 5'- gUCGGACGGCCUGAAUACCGAUGCGCUu -3' miRNA: 3'- -AGCCUGCCGGACUUAUGGCUACGCGA- -5' |
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28013 | 3' | -55.6 | NC_005887.1 | + | 39404 | 0.71 | 0.342392 |
Target: 5'- -aGGAUGGUCgcgGcGGUGCCGcgGCGCUu -3' miRNA: 3'- agCCUGCCGGa--C-UUAUGGCuaCGCGA- -5' |
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28013 | 3' | -55.6 | NC_005887.1 | + | 6435 | 0.73 | 0.27294 |
Target: 5'- cCGGACGGCCgcgccGAG-AUCGAagUGCGCg -3' miRNA: 3'- aGCCUGCCGGa----CUUaUGGCU--ACGCGa -5' |
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28013 | 3' | -55.6 | NC_005887.1 | + | 35076 | 0.77 | 0.130799 |
Target: 5'- gCGGGCGGCCgGG--GCCGAUGuCGCg -3' miRNA: 3'- aGCCUGCCGGaCUuaUGGCUAC-GCGa -5' |
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28013 | 3' | -55.6 | NC_005887.1 | + | 8846 | 0.66 | 0.614443 |
Target: 5'- gCGGGCaGCUugUGAAcGCCGgcGCGCc -3' miRNA: 3'- aGCCUGcCGG--ACUUaUGGCuaCGCGa -5' |
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28013 | 3' | -55.6 | NC_005887.1 | + | 20866 | 0.67 | 0.526293 |
Target: 5'- aCGGuguugGCGGCCUGAAUgauaguggcGCCGgcGUGUc -3' miRNA: 3'- aGCC-----UGCCGGACUUA---------UGGCuaCGCGa -5' |
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28013 | 3' | -55.6 | NC_005887.1 | + | 17744 | 0.69 | 0.463397 |
Target: 5'- cUCGGcACGGCCgcgcgGCUGAUGgCGCc -3' miRNA: 3'- -AGCC-UGCCGGacuuaUGGCUAC-GCGa -5' |
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28013 | 3' | -55.6 | NC_005887.1 | + | 10925 | 0.69 | 0.453294 |
Target: 5'- gUCGGcCGGCCUGcucacgcCCGAUuacGCGCa -3' miRNA: 3'- -AGCCuGCCGGACuuau---GGCUA---CGCGa -5' |
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28013 | 3' | -55.6 | NC_005887.1 | + | 2702 | 0.69 | 0.423724 |
Target: 5'- aCGGGCGGCaagcg-GCCGAgccGCGCg -3' miRNA: 3'- aGCCUGCCGgacuuaUGGCUa--CGCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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