Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28015 | 3' | -57 | NC_005887.1 | + | 30956 | 0.66 | 0.570967 |
Target: 5'- aUGuCGGcGCCCGcguacgugaggaacuUCGCCCACUguUCGg -3' miRNA: 3'- aGC-GCC-CGGGUu--------------AGUGGGUGAguAGC- -5' |
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28015 | 3' | -57 | NC_005887.1 | + | 17803 | 0.66 | 0.558994 |
Target: 5'- gUCGCaGGGgCUGAUCGCCgAgUCGcUCGa -3' miRNA: 3'- -AGCG-CCCgGGUUAGUGGgUgAGU-AGC- -5' |
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28015 | 3' | -57 | NC_005887.1 | + | 35721 | 0.66 | 0.537415 |
Target: 5'- aCGCGugcaGCUCGGUCgcggucgugGCCCACUCGcUCGg -3' miRNA: 3'- aGCGCc---CGGGUUAG---------UGGGUGAGU-AGC- -5' |
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28015 | 3' | -57 | NC_005887.1 | + | 41749 | 0.67 | 0.505615 |
Target: 5'- gCGCGGGCuCUggUCGaagaCCACg-AUCGa -3' miRNA: 3'- aGCGCCCG-GGuuAGUg---GGUGagUAGC- -5' |
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28015 | 3' | -57 | NC_005887.1 | + | 2904 | 0.67 | 0.505615 |
Target: 5'- aCGC-GGCCCuuGUCgAUCCACUCGUa- -3' miRNA: 3'- aGCGcCCGGGu-UAG-UGGGUGAGUAgc -5' |
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28015 | 3' | -57 | NC_005887.1 | + | 35408 | 0.67 | 0.495193 |
Target: 5'- cCGCGcacGGCCU--UCGCCCACgaGUCGa -3' miRNA: 3'- aGCGC---CCGGGuuAGUGGGUGagUAGC- -5' |
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28015 | 3' | -57 | NC_005887.1 | + | 24281 | 0.67 | 0.491052 |
Target: 5'- cUUGcCGGGCgucgccuuguucaCGAUCGCCCAaUCGUCGg -3' miRNA: 3'- -AGC-GCCCGg------------GUUAGUGGGUgAGUAGC- -5' |
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28015 | 3' | -57 | NC_005887.1 | + | 40974 | 0.67 | 0.48487 |
Target: 5'- gCGCGGGCCUGAUguacgUGCCCgaGCUCGa-- -3' miRNA: 3'- aGCGCCCGGGUUA-----GUGGG--UGAGUagc -5' |
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28015 | 3' | -57 | NC_005887.1 | + | 6649 | 0.67 | 0.48487 |
Target: 5'- cUCGUugugugGGGCCCGAauacCugCCACUCgAUCu -3' miRNA: 3'- -AGCG------CCCGGGUUa---GugGGUGAG-UAGc -5' |
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28015 | 3' | -57 | NC_005887.1 | + | 29926 | 0.67 | 0.48487 |
Target: 5'- cCGCGGGCCUcg-CGCCgAuCUCGggaUCGa -3' miRNA: 3'- aGCGCCCGGGuuaGUGGgU-GAGU---AGC- -5' |
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28015 | 3' | -57 | NC_005887.1 | + | 32103 | 0.67 | 0.48487 |
Target: 5'- -gGCGGcGgaCGAUCACCCACaggUCAUCa -3' miRNA: 3'- agCGCC-CggGUUAGUGGGUG---AGUAGc -5' |
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28015 | 3' | -57 | NC_005887.1 | + | 18391 | 0.67 | 0.46454 |
Target: 5'- cUCGCGaaCCCGAUgGCCUacgccgcggcGCUCGUCGu -3' miRNA: 3'- -AGCGCccGGGUUAgUGGG----------UGAGUAGC- -5' |
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28015 | 3' | -57 | NC_005887.1 | + | 9100 | 0.68 | 0.454543 |
Target: 5'- gCGCGGGCaCCGG-CGCCguUACUCugGUCGc -3' miRNA: 3'- aGCGCCCG-GGUUaGUGG--GUGAG--UAGC- -5' |
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28015 | 3' | -57 | NC_005887.1 | + | 1445 | 0.68 | 0.434906 |
Target: 5'- gCGCGGuaGCCCu-UCGgCgGCUCGUCGu -3' miRNA: 3'- aGCGCC--CGGGuuAGUgGgUGAGUAGC- -5' |
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28015 | 3' | -57 | NC_005887.1 | + | 13665 | 0.68 | 0.415769 |
Target: 5'- uUCGCGGGCgUGAUCGCgggUugUCGUCu -3' miRNA: 3'- -AGCGCCCGgGUUAGUGg--GugAGUAGc -5' |
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28015 | 3' | -57 | NC_005887.1 | + | 24165 | 0.68 | 0.414825 |
Target: 5'- cUCGCaGGaacGCCCGAUCgauagccGCCCACagCGUCGu -3' miRNA: 3'- -AGCG-CC---CGGGUUAG-------UGGGUGa-GUAGC- -5' |
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28015 | 3' | -57 | NC_005887.1 | + | 25780 | 0.69 | 0.361578 |
Target: 5'- cUUGCGGGCCCGGUCgagAUCgGCUUcgUGc -3' miRNA: 3'- -AGCGCCCGGGUUAG---UGGgUGAGuaGC- -5' |
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28015 | 3' | -57 | NC_005887.1 | + | 33946 | 0.71 | 0.297304 |
Target: 5'- gUCGUGcucGGCCCAcgCGCCUugUCGcuUCGu -3' miRNA: 3'- -AGCGC---CCGGGUuaGUGGGugAGU--AGC- -5' |
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28015 | 3' | -57 | NC_005887.1 | + | 34870 | 0.74 | 0.175847 |
Target: 5'- aUCGCGGcCUCGAUCGCCUugUCGUUc -3' miRNA: 3'- -AGCGCCcGGGUUAGUGGGugAGUAGc -5' |
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28015 | 3' | -57 | NC_005887.1 | + | 32549 | 1.08 | 0.000567 |
Target: 5'- uUCGCGGGCCCAAUCACCCACUCAUCGc -3' miRNA: 3'- -AGCGCCCGGGUUAGUGGGUGAGUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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