Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28021 | 5' | -56.8 | NC_005887.1 | + | 28371 | 1.05 | 0.000926 |
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Target: 5'- cUUCGUCGGGAACGUCAGGUGCACCGUg -3' miRNA: 3'- -AAGCAGCCCUUGCAGUCCACGUGGCA- -5' |
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| 28021 | 5' | -56.8 | NC_005887.1 | + | 17267 | 0.73 | 0.210863 |
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Target: 5'- aUCGUgcCGGGcgaGACGUCGGGcgGCGCCGc -3' miRNA: 3'- aAGCA--GCCC---UUGCAGUCCa-CGUGGCa -5' |
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| 28021 | 5' | -56.8 | NC_005887.1 | + | 39509 | 0.73 | 0.222451 |
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Target: 5'- ---aUUGGG-AUGUCGGGUGCACCGc -3' miRNA: 3'- aagcAGCCCuUGCAGUCCACGUGGCa -5' |
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| 28021 | 5' | -56.8 | NC_005887.1 | + | 10991 | 0.7 | 0.311369 |
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Target: 5'- cUCGUCGaGGAugGcguggugcUCGGGUGCGgCGg -3' miRNA: 3'- aAGCAGC-CCUugC--------AGUCCACGUgGCa -5' |
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| 28021 | 5' | -56.8 | NC_005887.1 | + | 28304 | 0.68 | 0.434544 |
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Target: 5'- ----cCGGGAACGgcaucgucUCAGGcUGCGCCGg -3' miRNA: 3'- aagcaGCCCUUGC--------AGUCC-ACGUGGCa -5' |
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| 28021 | 5' | -56.8 | NC_005887.1 | + | 24989 | 0.67 | 0.484886 |
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Target: 5'- --gGUCGGGGuaguugccgaGCGUCAGcucGCGCCGg -3' miRNA: 3'- aagCAGCCCU----------UGCAGUCca-CGUGGCa -5' |
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| 28021 | 5' | -56.8 | NC_005887.1 | + | 11030 | 0.66 | 0.516393 |
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Target: 5'- gUCGgcaCGuGGGACGUgAccaccGGUGCGCCGa -3' miRNA: 3'- aAGCa--GC-CCUUGCAgU-----CCACGUGGCa -5' |
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| 28021 | 5' | -56.8 | NC_005887.1 | + | 14199 | 0.66 | 0.559602 |
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Target: 5'- -cUGUCGGGcGCGgccucggaCAGGgcgGCGCCGc -3' miRNA: 3'- aaGCAGCCCuUGCa-------GUCCa--CGUGGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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