Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28022 | 5' | -57.1 | NC_005887.1 | + | 28212 | 1.07 | 0.000722 |
Target: 5'- cCGUCGGCUCGGGCACAUCGAUCUGGUu -3' miRNA: 3'- -GCAGCCGAGCCCGUGUAGCUAGACCA- -5' |
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28022 | 5' | -57.1 | NC_005887.1 | + | 11004 | 0.78 | 0.091482 |
Target: 5'- gCGUgGuGCUCGGGUGCggCGGUCUGGUc -3' miRNA: 3'- -GCAgC-CGAGCCCGUGuaGCUAGACCA- -5' |
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28022 | 5' | -57.1 | NC_005887.1 | + | 7227 | 0.76 | 0.135855 |
Target: 5'- uGUCGGCgUCGGGCGCAUCGAc----- -3' miRNA: 3'- gCAGCCG-AGCCCGUGUAGCUagacca -5' |
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28022 | 5' | -57.1 | NC_005887.1 | + | 29368 | 0.76 | 0.135855 |
Target: 5'- gGUCGGUgccgCGGGuCGCcgcuUCGGUCUGGUa -3' miRNA: 3'- gCAGCCGa---GCCC-GUGu---AGCUAGACCA- -5' |
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28022 | 5' | -57.1 | NC_005887.1 | + | 8617 | 0.74 | 0.193988 |
Target: 5'- cCGUU-GCUCGGuGCACguGUCGAUCUGGc -3' miRNA: 3'- -GCAGcCGAGCC-CGUG--UAGCUAGACCa -5' |
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28022 | 5' | -57.1 | NC_005887.1 | + | 14538 | 0.7 | 0.341136 |
Target: 5'- cCGUCGGC---GGCGCGUCGAUC-GGc -3' miRNA: 3'- -GCAGCCGagcCCGUGUAGCUAGaCCa -5' |
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28022 | 5' | -57.1 | NC_005887.1 | + | 8200 | 0.69 | 0.402339 |
Target: 5'- uGUCGGCUCuGGCAaGUCGGcccUCUGuGg -3' miRNA: 3'- gCAGCCGAGcCCGUgUAGCU---AGAC-Ca -5' |
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28022 | 5' | -57.1 | NC_005887.1 | + | 19796 | 0.68 | 0.450105 |
Target: 5'- aG-CGuCUCGGGCAUGUCGAUCgcGGa -3' miRNA: 3'- gCaGCcGAGCCCGUGUAGCUAGa-CCa -5' |
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28022 | 5' | -57.1 | NC_005887.1 | + | 34152 | 0.67 | 0.460029 |
Target: 5'- uGUCGGUgcgCGGGUagACAUCGAaCUGc- -3' miRNA: 3'- gCAGCCGa--GCCCG--UGUAGCUaGACca -5' |
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28022 | 5' | -57.1 | NC_005887.1 | + | 33906 | 0.67 | 0.480217 |
Target: 5'- uCGUCGuGCUCGGGagugaagccuuCGCGUCGAcCUGc- -3' miRNA: 3'- -GCAGC-CGAGCCC-----------GUGUAGCUaGACca -5' |
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28022 | 5' | -57.1 | NC_005887.1 | + | 27983 | 0.67 | 0.500828 |
Target: 5'- cCGcCGGCUCGGGCcgaaucGCGUCG-UC-GGc -3' miRNA: 3'- -GCaGCCGAGCCCG------UGUAGCuAGaCCa -5' |
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28022 | 5' | -57.1 | NC_005887.1 | + | 18159 | 0.67 | 0.509181 |
Target: 5'- aCGUCGGCaacacgacgcugUGGGCGgcUAUCGAUCgGGc -3' miRNA: 3'- -GCAGCCGa-----------GCCCGU--GUAGCUAGaCCa -5' |
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28022 | 5' | -57.1 | NC_005887.1 | + | 6945 | 0.66 | 0.540997 |
Target: 5'- uCGUgGGCgaaggccgugcgCGGGCGCAUCGAgcaugagCUGu- -3' miRNA: 3'- -GCAgCCGa-----------GCCCGUGUAGCUa------GACca -5' |
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28022 | 5' | -57.1 | NC_005887.1 | + | 40886 | 0.66 | 0.575633 |
Target: 5'- cCGUCGGCgUCGGGCuccugcucgcACAU-GAUC-GGa -3' miRNA: 3'- -GCAGCCG-AGCCCG----------UGUAgCUAGaCCa -5' |
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28022 | 5' | -57.1 | NC_005887.1 | + | 1369 | 0.66 | 0.575633 |
Target: 5'- -uUCGaGCUCGGGCACGUacAUCaGGc -3' miRNA: 3'- gcAGC-CGAGCCCGUGUAgcUAGaCCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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