Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28025 | 5' | -56.7 | NC_005887.1 | + | 22798 | 0.67 | 0.500969 |
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Target: 5'- cGGCCGCUGUUGUucacgAGcuacgCGGCGcAUCc- -3' miRNA: 3'- cCCGGCGGCAACA-----UCa----GCCGC-UAGua -5' |
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| 28025 | 5' | -56.7 | NC_005887.1 | + | 26208 | 0.66 | 0.532836 |
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Target: 5'- cGGCgCGCCG--GUGGUUGGCGGg--- -3' miRNA: 3'- cCCG-GCGGCaaCAUCAGCCGCUagua -5' |
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| 28025 | 5' | -56.7 | NC_005887.1 | + | 27122 | 1.07 | 0.000664 |
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Target: 5'- cGGGCCGCCGUUGUAGUCGGCGAUCAUc -3' miRNA: 3'- -CCCGGCGGCAACAUCAGCCGCUAGUA- -5' |
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| 28025 | 5' | -56.7 | NC_005887.1 | + | 32339 | 0.86 | 0.027811 |
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Target: 5'- cGGCCGCgCGUgaUGUGGUCGGCGAUCu- -3' miRNA: 3'- cCCGGCG-GCA--ACAUCAGCCGCUAGua -5' |
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| 28025 | 5' | -56.7 | NC_005887.1 | + | 32422 | 0.73 | 0.232165 |
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Target: 5'- uGGCCGgCGgcgaUGUGGccggCGGCGAUCAg -3' miRNA: 3'- cCCGGCgGCa---ACAUCa---GCCGCUAGUa -5' |
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| 28025 | 5' | -56.7 | NC_005887.1 | + | 35060 | 0.66 | 0.554479 |
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Target: 5'- aGGUCGCUGccGUcGUCGGCGggCGc -3' miRNA: 3'- cCCGGCGGCaaCAuCAGCCGCuaGUa -5' |
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| 28025 | 5' | -56.7 | NC_005887.1 | + | 37834 | 0.77 | 0.12004 |
|
Target: 5'- cGGGaCGCCGccGUAGUCGGCGAgCAg -3' miRNA: 3'- -CCCgGCGGCaaCAUCAGCCGCUaGUa -5' |
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| 28025 | 5' | -56.7 | NC_005887.1 | + | 40778 | 0.66 | 0.576373 |
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Target: 5'- -cGCCGCCG----AGcCGGCGGUCGa -3' miRNA: 3'- ccCGGCGGCaacaUCaGCCGCUAGUa -5' |
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| 28025 | 5' | -56.7 | NC_005887.1 | + | 41363 | 0.7 | 0.323867 |
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Target: 5'- cGGCgGCCGgcaauUUGcgcguGUCGGCGAUCAc -3' miRNA: 3'- cCCGgCGGC-----AACau---CAGCCGCUAGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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