Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28027 | 3' | -56.4 | NC_005887.1 | + | 39835 | 0.71 | 0.304829 |
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Target: 5'- gCAGcgUGCGGCaGuCCgggugcaCGAGCAGGCCg -3' miRNA: 3'- -GUCaaGCGCCG-CuGGa------GCUUGUCCGG- -5' |
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| 28027 | 3' | -56.4 | NC_005887.1 | + | 40155 | 0.7 | 0.360718 |
|
Target: 5'- uCGGUUUGCGcugcccgaucaccGCGGCCggCGu-CAGGCCg -3' miRNA: 3'- -GUCAAGCGC-------------CGCUGGa-GCuuGUCCGG- -5' |
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| 28027 | 3' | -56.4 | NC_005887.1 | + | 40333 | 0.67 | 0.537415 |
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Target: 5'- -cGUUCGCcGCcgaaauggacuGGCCgUCGAGCGcGGCCg -3' miRNA: 3'- guCAAGCGcCG-----------CUGG-AGCUUGU-CCGG- -5' |
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| 28027 | 3' | -56.4 | NC_005887.1 | + | 40798 | 0.67 | 0.537415 |
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Target: 5'- aGGUUCGUGGCGGCgacgcgccgCUCGAcgaugACgAGGUg -3' miRNA: 3'- gUCAAGCGCCGCUG---------GAGCU-----UG-UCCGg -5' |
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| 28027 | 3' | -56.4 | NC_005887.1 | + | 40813 | 0.66 | 0.613832 |
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Target: 5'- cCAGUccuUCGCGGU--CgUCGAcaGCAGGUCc -3' miRNA: 3'- -GUCA---AGCGCCGcuGgAGCU--UGUCCGG- -5' |
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| 28027 | 3' | -56.4 | NC_005887.1 | + | 41523 | 0.68 | 0.474651 |
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Target: 5'- --cUUCGCGGCcgcGAUCgcgCGAGCAuGGCUg -3' miRNA: 3'- gucAAGCGCCG---CUGGa--GCUUGU-CCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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