Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28027 | 3' | -56.4 | NC_005887.1 | + | 1592 | 0.75 | 0.169715 |
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Target: 5'- gCGGcgCGCGGCGcgccaacggcgaucGCCUCGAcGCGGGCg -3' miRNA: 3'- -GUCaaGCGCCGC--------------UGGAGCU-UGUCCGg -5' |
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| 28027 | 3' | -56.4 | NC_005887.1 | + | 14201 | 0.76 | 0.145038 |
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Target: 5'- ---gUCGgGcGCGGCCUCGGACAGGgCg -3' miRNA: 3'- gucaAGCgC-CGCUGGAGCUUGUCCgG- -5' |
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| 28027 | 3' | -56.4 | NC_005887.1 | + | 20443 | 0.77 | 0.122295 |
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Target: 5'- aCAGUcgUCGUGGCGGCaagCGAgauacgcGCGGGCCa -3' miRNA: 3'- -GUCA--AGCGCCGCUGga-GCU-------UGUCCGG- -5' |
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| 28027 | 3' | -56.4 | NC_005887.1 | + | 1591 | 0.78 | 0.103488 |
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Target: 5'- gGGUggGUGGCGGCCUCGAACc-GCCa -3' miRNA: 3'- gUCAagCGCCGCUGGAGCUUGucCGG- -5' |
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| 28027 | 3' | -56.4 | NC_005887.1 | + | 343 | 0.81 | 0.07124 |
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Target: 5'- ----cCGCGGCGACaCUCGAcgugggccGCAGGCCg -3' miRNA: 3'- gucaaGCGCCGCUG-GAGCU--------UGUCCGG- -5' |
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| 28027 | 3' | -56.4 | NC_005887.1 | + | 26025 | 1.12 | 0.000372 |
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Target: 5'- gCAGUUCGCGGCGACCUCGAACAGGCCg -3' miRNA: 3'- -GUCAAGCGCCGCUGGAGCUUGUCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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