miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
28028 3' -54.7 NC_005887.1 + 30341 0.7 0.395802
Target:  5'- -cGCGCAgCCGGUuuucaaugccCCGaUGCCGAGCu -3'
miRNA:   3'- gaCGCGUaGGCUA----------GGCaACGGUUCGu -5'
28028 3' -54.7 NC_005887.1 + 35586 0.7 0.394863
Target:  5'- gUGCGUgcgguaaccuuccAUCCGAccgCCGUUGCCGuccGCAu -3'
miRNA:   3'- gACGCG-------------UAGGCUa--GGCAACGGUu--CGU- -5'
28028 3' -54.7 NC_005887.1 + 24900 0.71 0.350632
Target:  5'- uUGCGCGUCgGAUUCGg-GCCGuAGCGc -3'
miRNA:   3'- gACGCGUAGgCUAGGCaaCGGU-UCGU- -5'
28028 3' -54.7 NC_005887.1 + 26942 0.72 0.309248
Target:  5'- aUGCGCGUCgaCGAUCCGcUGCCcgcGCGc -3'
miRNA:   3'- gACGCGUAG--GCUAGGCaACGGuu-CGU- -5'
28028 3' -54.7 NC_005887.1 + 26845 0.74 0.237893
Target:  5'- -gGCGCGUCUGccgguagCCGUUGCCGccgGGCAc -3'
miRNA:   3'- gaCGCGUAGGCua-----GGCAACGGU---UCGU- -5'
28028 3' -54.7 NC_005887.1 + 23730 0.75 0.202054
Target:  5'- -cGgGCcgCCGAUCgCGcUGCCGAGCAa -3'
miRNA:   3'- gaCgCGuaGGCUAG-GCaACGGUUCGU- -5'
28028 3' -54.7 NC_005887.1 + 25320 1.08 0.000817
Target:  5'- gCUGCGCAUCCGAUCCGUUGCCAAGCAc -3'
miRNA:   3'- -GACGCGUAGGCUAGGCAACGGUUCGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.