Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28029 | 5' | -57.4 | NC_005887.1 | + | 36870 | 0.71 | 0.256447 |
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Target: 5'- -gCCGGUGCuGCGCCaucGGCCuGCGUCGUg -3' miRNA: 3'- aaGGUCAUG-CGCGG---CUGGcUGCAGUA- -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 18404 | 0.71 | 0.239534 |
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Target: 5'- -gUCGGUGCGUGCgcagacggccgcgaGGCCGACGUCAUc -3' miRNA: 3'- aaGGUCAUGCGCGg-------------CUGGCUGCAGUA- -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 26112 | 0.71 | 0.23082 |
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Target: 5'- -gCCcGUGCGCucGCCGGCCGGCGUa-- -3' miRNA: 3'- aaGGuCAUGCG--CGGCUGGCUGCAgua -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 9977 | 0.72 | 0.207386 |
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Target: 5'- gUCCAcaacUACGUGUCGACgGACGUCAc -3' miRNA: 3'- aAGGUc---AUGCGCGGCUGgCUGCAGUa -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 31263 | 0.73 | 0.191186 |
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Target: 5'- -aCCAGUACuCGUCGGCCG-CGUCGa -3' miRNA: 3'- aaGGUCAUGcGCGGCUGGCuGCAGUa -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 31318 | 0.73 | 0.186038 |
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Target: 5'- -aCCGGUggucacgucccACGUGCCGACCGGgcCGUCGUc -3' miRNA: 3'- aaGGUCA-----------UGCGCGGCUGGCU--GCAGUA- -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 35479 | 0.74 | 0.149095 |
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Target: 5'- -aUCAGcgccUGCGCGuuGGCCGACGUCGc -3' miRNA: 3'- aaGGUC----AUGCGCggCUGGCUGCAGUa -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 24806 | 1.04 | 0.000954 |
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Target: 5'- gUUCCAGUACGCGCCGACCGACGUCAUg -3' miRNA: 3'- -AAGGUCAUGCGCGGCUGGCUGCAGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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