Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28030 | 5' | -55.7 | NC_005887.1 | + | 31933 | 0.69 | 0.423276 |
Target: 5'- uCAGaCCGCGACCGuaacgCCUUCGAGg -3' miRNA: 3'- uGUC-GGUGCUGGCucucaGGAAGCUU- -5' |
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28030 | 5' | -55.7 | NC_005887.1 | + | 24573 | 0.93 | 0.009278 |
Target: 5'- gACAGCCACGACCGGaAGUCCUUCGAAc -3' miRNA: 3'- -UGUCGGUGCUGGCUcUCAGGAAGCUU- -5' |
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28030 | 5' | -55.7 | NC_005887.1 | + | 30532 | 0.68 | 0.440014 |
Target: 5'- gGCGGCCGuCGGCCGAGAcgccacgcagcagcGUCuuguaCUUCGAc -3' miRNA: 3'- -UGUCGGU-GCUGGCUCU--------------CAG-----GAAGCUu -5' |
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28030 | 5' | -55.7 | NC_005887.1 | + | 24679 | 0.67 | 0.515828 |
Target: 5'- cCAGCCGCGGCCGGca-UCCU-CGAc -3' miRNA: 3'- uGUCGGUGCUGGCUcucAGGAaGCUu -5' |
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28030 | 5' | -55.7 | NC_005887.1 | + | 29422 | 0.66 | 0.559553 |
Target: 5'- gACAGCUcguCGGCCGAGuAGUCg-UCGGc -3' miRNA: 3'- -UGUCGGu--GCUGGCUC-UCAGgaAGCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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