Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28032 | 3' | -53 | NC_005887.1 | + | 14470 | 0.71 | 0.436772 |
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Target: 5'- aGGCgucgUGUCgggCCUCGCGcaGAUCGUGCCc -3' miRNA: 3'- -UUGa---GCGGaa-GGAGCGC--UUAGUACGG- -5' |
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| 28032 | 3' | -53 | NC_005887.1 | + | 29837 | 0.72 | 0.378735 |
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Target: 5'- gAGCuUCGCCUUCgUCuGCGGAUCcagcGCCg -3' miRNA: 3'- -UUG-AGCGGAAGgAG-CGCUUAGua--CGG- -5' |
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| 28032 | 3' | -53 | NC_005887.1 | + | 23171 | 0.73 | 0.368671 |
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Target: 5'- uGGCUUGCCUUUCUCGuCGcgcucgcGAUCAcgGCCg -3' miRNA: 3'- -UUGAGCGGAAGGAGC-GC-------UUAGUa-CGG- -5' |
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| 28032 | 3' | -53 | NC_005887.1 | + | 816 | 0.79 | 0.139231 |
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Target: 5'- uGCgCGCCgagcgCCUCGCGGAUCuUGCCg -3' miRNA: 3'- uUGaGCGGaa---GGAGCGCUUAGuACGG- -5' |
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| 28032 | 3' | -53 | NC_005887.1 | + | 30264 | 0.81 | 0.107028 |
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Target: 5'- cGCUUGCCgUCCggCGCGAcgAUCGUGCCg -3' miRNA: 3'- uUGAGCGGaAGGa-GCGCU--UAGUACGG- -5' |
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| 28032 | 3' | -53 | NC_005887.1 | + | 24170 | 1.11 | 0.000815 |
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Target: 5'- gAACUCGCCUUCCUCGCGAAUCAUGCCg -3' miRNA: 3'- -UUGAGCGGAAGGAGCGCUUAGUACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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