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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 28032 | 5' | -64.1 | NC_005887.1 | + | 28365 | 0.66 | 0.266965 |
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Target: 5'- uUCGaGCACGaacGCGUCGaCGGCUuCGCgGCUg -3' miRNA: 3'- -AGC-CGUGC---UGCAGCcGCCGG-GCG-CGA- -5' |
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| 28032 | 5' | -64.1 | NC_005887.1 | + | 10377 | 0.66 | 0.266965 |
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Target: 5'- aCGGCGugcCGACGUCGacuaucgcCGGCCuCGgGCa -3' miRNA: 3'- aGCCGU---GCUGCAGCc-------GCCGG-GCgCGa -5' |
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| 28032 | 5' | -64.1 | NC_005887.1 | + | 33833 | 0.66 | 0.254208 |
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Target: 5'- gUCGGCGCGGCGaUCgaacgcaugcagGGCGuacgaGCCUGCGg- -3' miRNA: 3'- -AGCCGUGCUGC-AG------------CCGC-----CGGGCGCga -5' |
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| 28032 | 5' | -64.1 | NC_005887.1 | + | 18937 | 0.66 | 0.254208 |
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Target: 5'- cUCGcaGCGGcCGUCGGCGGCCaauaCGCa -3' miRNA: 3'- -AGCcgUGCU-GCAGCCGCCGGgc--GCGa -5' |
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| 28032 | 5' | -64.1 | NC_005887.1 | + | 38468 | 0.66 | 0.241957 |
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Target: 5'- cCGGCAUGGCGUCGuaugcCGGgCUGcCGCc -3' miRNA: 3'- aGCCGUGCUGCAGCc----GCCgGGC-GCGa -5' |
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| 28032 | 5' | -64.1 | NC_005887.1 | + | 13493 | 0.65 | 0.27821 |
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Target: 5'- cCGGCgcaucuuuccgaaaACGAUGUCuGGCGuCCCGgGCc -3' miRNA: 3'- aGCCG--------------UGCUGCAG-CCGCcGGGCgCGa -5' |
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| 28032 | 5' | -64.1 | NC_005887.1 | + | 24501 | 0.65 | 0.279558 |
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Target: 5'- gUCGuCGCGAgguUGUCGGUGccccaccgauugaGCCCGUGCUc -3' miRNA: 3'- -AGCcGUGCU---GCAGCCGC-------------CGGGCGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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