Results 121 - 127 of 127 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28032 | 5' | -64.1 | NC_005887.1 | + | 40554 | 0.66 | 0.24802 |
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Target: 5'- gCGGCACGuccuuCGgguaGCGGCCgaCGCGCa -3' miRNA: 3'- aGCCGUGCu----GCagc-CGCCGG--GCGCGa -5' |
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| 28032 | 5' | -64.1 | NC_005887.1 | + | 40753 | 0.69 | 0.169455 |
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Target: 5'- -aGGCGauCGcCGUUGGCGcGCCgCGCGCc -3' miRNA: 3'- agCCGU--GCuGCAGCCGC-CGG-GCGCGa -5' |
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| 28032 | 5' | -64.1 | NC_005887.1 | + | 40788 | 0.66 | 0.241957 |
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Target: 5'- cCGGCGguCGAgGUucgUGGCGGCgaCGCGCc -3' miRNA: 3'- aGCCGU--GCUgCA---GCCGCCGg-GCGCGa -5' |
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| 28032 | 5' | -64.1 | NC_005887.1 | + | 41590 | 0.8 | 0.021538 |
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Target: 5'- gUCGGCcaGCGGCGgcccaggugCGGCGGCCgGCGCg -3' miRNA: 3'- -AGCCG--UGCUGCa--------GCCGCCGGgCGCGa -5' |
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| 28032 | 5' | -64.1 | NC_005887.1 | + | 41658 | 0.67 | 0.2151 |
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Target: 5'- gUCGGCGagcagcaucgacuCGGCGagcagcagcaccuggUCGGCGGUCaUGCGCg -3' miRNA: 3'- -AGCCGU-------------GCUGC---------------AGCCGCCGG-GCGCGa -5' |
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| 28032 | 5' | -64.1 | NC_005887.1 | + | 41815 | 0.82 | 0.016694 |
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Target: 5'- cCGGCGCGGCGcgCGGCGGCCgaCGCGUUc -3' miRNA: 3'- aGCCGUGCUGCa-GCCGCCGG--GCGCGA- -5' |
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| 28032 | 5' | -64.1 | NC_005887.1 | + | 41944 | 0.68 | 0.192807 |
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Target: 5'- gCGGCGCGGCGcgucuucgCGGCGcuuGCCCGUcuuuucGCa -3' miRNA: 3'- aGCCGUGCUGCa-------GCCGC---CGGGCG------CGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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