Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28035 | 3' | -56 | NC_005887.1 | + | 36832 | 0.66 | 0.552013 |
Target: 5'- -gCGUGCCugcugcgGCCGGUGCugCGG--CCGg -3' miRNA: 3'- caGCACGG-------CGGUUACGugGUCuaGGC- -5' |
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28035 | 3' | -56 | NC_005887.1 | + | 27195 | 0.66 | 0.557505 |
Target: 5'- uUCGccaUGCCGUCGA-GCGCCugcauggggucggucGGGUCCGu -3' miRNA: 3'- cAGC---ACGGCGGUUaCGUGG---------------UCUAGGC- -5' |
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28035 | 3' | -56 | NC_005887.1 | + | 34850 | 0.66 | 0.607504 |
Target: 5'- gGUCGUGCCGCCGaggGUGUaguucaaGCgCAGGUagCGc -3' miRNA: 3'- -CAGCACGGCGGU---UACG-------UG-GUCUAg-GC- -5' |
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28035 | 3' | -56 | NC_005887.1 | + | 2080 | 0.66 | 0.58629 |
Target: 5'- cGUCGacgGCaaGCCGGUGCACCcggacuaugcGGAUUCGc -3' miRNA: 3'- -CAGCa--CGg-CGGUUACGUGG----------UCUAGGC- -5' |
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28035 | 3' | -56 | NC_005887.1 | + | 4617 | 0.66 | 0.55311 |
Target: 5'- ---aUGCuCGCCGcgGCGCCGGuggCCGc -3' miRNA: 3'- cagcACG-GCGGUuaCGUGGUCua-GGC- -5' |
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28035 | 3' | -56 | NC_005887.1 | + | 22424 | 0.66 | 0.564116 |
Target: 5'- cGUCGUGCUGCUGuucUGCAUguGGauugCCGu -3' miRNA: 3'- -CAGCACGGCGGUu--ACGUGguCUa---GGC- -5' |
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28035 | 3' | -56 | NC_005887.1 | + | 16453 | 0.66 | 0.57407 |
Target: 5'- -gCGUGCgCGCgGgcAUGCGCUcgaccgcgcggcaGGAUCCGg -3' miRNA: 3'- caGCACG-GCGgU--UACGUGG-------------UCUAGGC- -5' |
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28035 | 3' | -56 | NC_005887.1 | + | 4897 | 0.66 | 0.575178 |
Target: 5'- gGUCGccgaggGCCGgCGcgagguguucGUGCGCCuGAUCCa -3' miRNA: 3'- -CAGCa-----CGGCgGU----------UACGUGGuCUAGGc -5' |
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28035 | 3' | -56 | NC_005887.1 | + | 11043 | 0.67 | 0.520513 |
Target: 5'- -aCGUGaCCaCCGGUGCGCC-GAUgCCGg -3' miRNA: 3'- caGCAC-GGcGGUUACGUGGuCUA-GGC- -5' |
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28035 | 3' | -56 | NC_005887.1 | + | 31984 | 0.67 | 0.520513 |
Target: 5'- cGUCG-GCaCGCCGuUGUACguGAUaCCGg -3' miRNA: 3'- -CAGCaCG-GCGGUuACGUGguCUA-GGC- -5' |
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28035 | 3' | -56 | NC_005887.1 | + | 13998 | 0.67 | 0.531301 |
Target: 5'- uUCGUGCUcgaaGCCAAUGCcgcGCCGGcgcagacgCCGa -3' miRNA: 3'- cAGCACGG----CGGUUACG---UGGUCua------GGC- -5' |
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28035 | 3' | -56 | NC_005887.1 | + | 19227 | 0.67 | 0.499204 |
Target: 5'- ----cGUCGUguAUGCACCGGGUUCGa -3' miRNA: 3'- cagcaCGGCGguUACGUGGUCUAGGC- -5' |
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28035 | 3' | -56 | NC_005887.1 | + | 12132 | 0.67 | 0.531301 |
Target: 5'- -aCGagcagGCCGCCGucgcGCGCCAGAUUgGu -3' miRNA: 3'- caGCa----CGGCGGUua--CGUGGUCUAGgC- -5' |
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28035 | 3' | -56 | NC_005887.1 | + | 34643 | 0.67 | 0.542169 |
Target: 5'- cGUCGacgagGCgCGCCGGcuUGCcgguuuCCGGAUCCGc -3' miRNA: 3'- -CAGCa----CG-GCGGUU--ACGu-----GGUCUAGGC- -5' |
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28035 | 3' | -56 | NC_005887.1 | + | 38870 | 0.68 | 0.457819 |
Target: 5'- cGUCGUGCuCGaucauCAGcGCGCCAGGcCCGa -3' miRNA: 3'- -CAGCACG-GCg----GUUaCGUGGUCUaGGC- -5' |
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28035 | 3' | -56 | NC_005887.1 | + | 19498 | 0.68 | 0.488695 |
Target: 5'- -cCGgcgGCCGUCGAUgGUACCgAGAUCUGc -3' miRNA: 3'- caGCa--CGGCGGUUA-CGUGG-UCUAGGC- -5' |
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28035 | 3' | -56 | NC_005887.1 | + | 9089 | 0.68 | 0.488695 |
Target: 5'- --gGUGCaUGCCAGcgcggGCACCGGcgCCGu -3' miRNA: 3'- cagCACG-GCGGUUa----CGUGGUCuaGGC- -5' |
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28035 | 3' | -56 | NC_005887.1 | + | 6607 | 0.68 | 0.467997 |
Target: 5'- aUCGa--CGCUGGcgGCACCGGAUCCGa -3' miRNA: 3'- cAGCacgGCGGUUa-CGUGGUCUAGGC- -5' |
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28035 | 3' | -56 | NC_005887.1 | + | 813 | 0.68 | 0.44776 |
Target: 5'- -gCGUGCgCGCCGA-GCGCCucgcGGAUCUu -3' miRNA: 3'- caGCACG-GCGGUUaCGUGG----UCUAGGc -5' |
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28035 | 3' | -56 | NC_005887.1 | + | 12561 | 0.69 | 0.428017 |
Target: 5'- cGUCGacgGCCGCCGgcAUGCGCaUGGuGUCCGc -3' miRNA: 3'- -CAGCa--CGGCGGU--UACGUG-GUC-UAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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