miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
28038 5' -51.8 NC_005887.1 + 40819 0.66 0.817255
Target:  5'- cUUCGCGGUCGUCGacaGCagG-UCCGGg -3'
miRNA:   3'- cAAGUGCCAGUAGCa--CGagCaAGGUU- -5'
28038 5' -51.8 NC_005887.1 + 36717 0.66 0.796221
Target:  5'- -gUCACGucGUCGUCGUGCgaaacgauguuaaUCGUgCCGAu -3'
miRNA:   3'- caAGUGC--CAGUAGCACG-------------AGCAaGGUU- -5'
28038 5' -51.8 NC_005887.1 + 26900 0.66 0.776464
Target:  5'- --gCGCGGUCAUCGcgGCgaCGUcgUCCGGu -3'
miRNA:   3'- caaGUGCCAGUAGCa-CGa-GCA--AGGUU- -5'
28038 5' -51.8 NC_005887.1 + 24719 0.67 0.755021
Target:  5'- cUUCACGGcgcugacCGUCGUGCgcagCGUuUCCAu -3'
miRNA:   3'- cAAGUGCCa------GUAGCACGa---GCA-AGGUu -5'
28038 5' -51.8 NC_005887.1 + 33893 0.67 0.744084
Target:  5'- -gUCGCcguauuGGUCGUCGUGCUCGggagugaagCCu- -3'
miRNA:   3'- caAGUG------CCAGUAGCACGAGCaa-------GGuu -5'
28038 5' -51.8 NC_005887.1 + 41210 0.68 0.664746
Target:  5'- --cCGCGcGUCGUCGUGCcCGUgaCCGAg -3'
miRNA:   3'- caaGUGC-CAGUAGCACGaGCAa-GGUU- -5'
28038 5' -51.8 NC_005887.1 + 20393 1.07 0.002051
Target:  5'- cGUUCACGGUCAUCGUGCUCGUUCCAAa -3'
miRNA:   3'- -CAAGUGCCAGUAGCACGAGCAAGGUU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.