Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28040 | 5' | -53.6 | NC_005887.1 | + | 32819 | 0.74 | 0.281871 |
Target: 5'- -cACGAaCAGCGGGCCGaCGUGAUGg -3' miRNA: 3'- gcUGCUaGUCGCCCGGUgGCAUUAUa -5' |
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28040 | 5' | -53.6 | NC_005887.1 | + | 35063 | 0.75 | 0.267455 |
Target: 5'- aCGACGAcccaCAGCGGGCgGCCGgggccGAUGUc -3' miRNA: 3'- -GCUGCUa---GUCGCCCGgUGGCa----UUAUA- -5' |
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28040 | 5' | -53.6 | NC_005887.1 | + | 23720 | 0.75 | 0.240419 |
Target: 5'- uCGACag-CAGCGGGCCGCCGa----- -3' miRNA: 3'- -GCUGcuaGUCGCCCGGUGGCauuaua -5' |
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28040 | 5' | -53.6 | NC_005887.1 | + | 20130 | 1.07 | 0.001516 |
Target: 5'- gCGACGAUCAGCGGGCCACCGUAAUAUg -3' miRNA: 3'- -GCUGCUAGUCGCCCGGUGGCAUUAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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