Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28048 | 5' | -56.7 | NC_005887.1 | + | 26471 | 0.67 | 0.463246 |
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Target: 5'- cGAGCG-CACCGaGCUGCugGUGCGCCUg -3' miRNA: 3'- -CUCGUgGUGGUaCGGUG--UAUGCGGGa -5' |
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| 28048 | 5' | -56.7 | NC_005887.1 | + | 33792 | 0.67 | 0.463246 |
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Target: 5'- cGGUGCUGCCuu-CgGCAUGCGCCCUc -3' miRNA: 3'- cUCGUGGUGGuacGgUGUAUGCGGGA- -5' |
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| 28048 | 5' | -56.7 | NC_005887.1 | + | 23374 | 0.67 | 0.463246 |
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Target: 5'- -uGCGCCAUgcUGCCGCGU--GCCCUu -3' miRNA: 3'- cuCGUGGUGguACGGUGUAugCGGGA- -5' |
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| 28048 | 5' | -56.7 | NC_005887.1 | + | 25152 | 0.67 | 0.453065 |
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Target: 5'- -uGCGCgGCgCAacauUGCCGCGUGCGgCCg -3' miRNA: 3'- cuCGUGgUG-GU----ACGGUGUAUGCgGGa -5' |
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| 28048 | 5' | -56.7 | NC_005887.1 | + | 8657 | 0.67 | 0.453065 |
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Target: 5'- -cGCGCCAcgaaaaaccaauCCGgaGCCugAUAUGCCCg -3' miRNA: 3'- cuCGUGGU------------GGUa-CGGugUAUGCGGGa -5' |
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| 28048 | 5' | -56.7 | NC_005887.1 | + | 34763 | 0.67 | 0.453065 |
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Target: 5'- cGGCGCCGCUuacgcgAUGCCGuCcggAUACGCCUg -3' miRNA: 3'- cUCGUGGUGG------UACGGU-G---UAUGCGGGa -5' |
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| 28048 | 5' | -56.7 | NC_005887.1 | + | 28929 | 0.67 | 0.450034 |
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Target: 5'- -uGCGCCGCuCGucgcagcagcggcuUGCCGCcgACGUCCg -3' miRNA: 3'- cuCGUGGUG-GU--------------ACGGUGuaUGCGGGa -5' |
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| 28048 | 5' | -56.7 | NC_005887.1 | + | 4170 | 0.68 | 0.41361 |
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Target: 5'- uGAGC-CgGCCAUGCUGCAcacCGCCUa -3' miRNA: 3'- -CUCGuGgUGGUACGGUGUau-GCGGGa -5' |
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| 28048 | 5' | -56.7 | NC_005887.1 | + | 11220 | 0.66 | 0.537538 |
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Target: 5'- -cGCACgGCC-UGCCcgGCAagcuCGCCCUg -3' miRNA: 3'- cuCGUGgUGGuACGG--UGUau--GCGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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