Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28051 | 5' | -56.6 | NC_005887.1 | + | 37125 | 0.66 | 0.540507 |
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Target: 5'- --cAGCGaCaGGcUCguGUCGGUGCCCu -3' miRNA: 3'- uccUUGC-GaCCaAGguCAGCCACGGG- -5' |
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| 28051 | 5' | -56.6 | NC_005887.1 | + | 10279 | 0.67 | 0.498069 |
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Target: 5'- uGGAcuACGCcuaugGGUgugucccguuUCCGucugcgggcGUCGGUGCCCc -3' miRNA: 3'- uCCU--UGCGa----CCA----------AGGU---------CAGCCACGGG- -5' |
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| 28051 | 5' | -56.6 | NC_005887.1 | + | 27208 | 0.68 | 0.477412 |
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Target: 5'- -cGAGCGCcugcaUGGggUCGGUCGG-GUCCg -3' miRNA: 3'- ucCUUGCG-----ACCaaGGUCAGCCaCGGG- -5' |
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| 28051 | 5' | -56.6 | NC_005887.1 | + | 37788 | 0.68 | 0.467243 |
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Target: 5'- cGGcuUGCUGcgccugcaGUUCCucGUCGGUGCUCa -3' miRNA: 3'- uCCuuGCGAC--------CAAGGu-CAGCCACGGG- -5' |
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| 28051 | 5' | -56.6 | NC_005887.1 | + | 11394 | 0.68 | 0.447248 |
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Target: 5'- cGGAGCGCUGGcggCCGG-CGccGCCa -3' miRNA: 3'- uCCUUGCGACCaa-GGUCaGCcaCGGg -5' |
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| 28051 | 5' | -56.6 | NC_005887.1 | + | 24361 | 0.71 | 0.284189 |
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Target: 5'- cGGucAgGCU-GUUCCAGUCGGUGCggCCg -3' miRNA: 3'- uCCu-UgCGAcCAAGGUCAGCCACG--GG- -5' |
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| 28051 | 5' | -56.6 | NC_005887.1 | + | 29350 | 0.74 | 0.202232 |
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Target: 5'- cGGAAgcCGcCUGGUcCgGGUCGGUGCCg -3' miRNA: 3'- uCCUU--GC-GACCAaGgUCAGCCACGGg -5' |
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| 28051 | 5' | -56.6 | NC_005887.1 | + | 15476 | 1.12 | 0.000307 |
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Target: 5'- gAGGAACGCUGGUUCCAGUCGGUGCCCg -3' miRNA: 3'- -UCCUUGCGACCAAGGUCAGCCACGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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