Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28060 | 5' | -57.5 | NC_005887.1 | + | 29040 | 0.66 | 0.468879 |
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Target: 5'- -cGGCCggcguuGCCGAAcgccuGGcCGAUCUGGUUCAg -3' miRNA: 3'- uuCCGG------CGGUUU-----UC-GCUGGACCAGGU- -5' |
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| 28060 | 5' | -57.5 | NC_005887.1 | + | 36395 | 0.66 | 0.43857 |
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Target: 5'- -cGGCCGCgAu-GGUGGUCUGGUUCAu -3' miRNA: 3'- uuCCGGCGgUuuUCGCUGGACCAGGU- -5' |
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| 28060 | 5' | -57.5 | NC_005887.1 | + | 41465 | 0.67 | 0.418032 |
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Target: 5'- cGAGGCCGCCuGAcucguccGGCGGCgCgGG-CCAc -3' miRNA: 3'- -UUCCGGCGGuUU-------UCGCUG-GaCCaGGU- -5' |
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| 28060 | 5' | -57.5 | NC_005887.1 | + | 37699 | 0.67 | 0.399964 |
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Target: 5'- cAAGcGaCGCCGGGGGCGAUCaGGUUCAg -3' miRNA: 3'- -UUC-CgGCGGUUUUCGCUGGaCCAGGU- -5' |
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| 28060 | 5' | -57.5 | NC_005887.1 | + | 26670 | 0.7 | 0.283084 |
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Target: 5'- -cGGCCGCgCGGcGGUGAgCUGGUCgAg -3' miRNA: 3'- uuCCGGCG-GUUuUCGCUgGACCAGgU- -5' |
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| 28060 | 5' | -57.5 | NC_005887.1 | + | 29338 | 0.7 | 0.283084 |
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Target: 5'- -cGaGCUGCUucacGGAAGCcGCCUGGUCCGg -3' miRNA: 3'- uuC-CGGCGG----UUUUCGcUGGACCAGGU- -5' |
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| 28060 | 5' | -57.5 | NC_005887.1 | + | 25088 | 0.7 | 0.26185 |
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Target: 5'- uGAGGCCGCCGccgucgGAuuucGCGACCgguuGUCCGg -3' miRNA: 3'- -UUCCGGCGGU------UUu---CGCUGGac--CAGGU- -5' |
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| 28060 | 5' | -57.5 | NC_005887.1 | + | 27547 | 0.73 | 0.165122 |
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Target: 5'- -cGGCCGCCAGAu-UGAUCgGGUCCAg -3' miRNA: 3'- uuCCGGCGGUUUucGCUGGaCCAGGU- -5' |
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| 28060 | 5' | -57.5 | NC_005887.1 | + | 13364 | 1.06 | 0.000573 |
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Target: 5'- gAAGGCCGCCAAAAGCGACCUGGUCCAc -3' miRNA: 3'- -UUCCGGCGGUUUUCGCUGGACCAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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