Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2807 | 3' | -60.3 | NC_001491.2 | + | 82926 | 0.66 | 0.632482 |
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Target: 5'- gUCCUGCGguuGGGGCacggGCUGCUGGu--- -3' miRNA: 3'- uGGGACGCg--UCCCG----CGACGACCuaaa -5' |
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| 2807 | 3' | -60.3 | NC_001491.2 | + | 83587 | 0.66 | 0.622266 |
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Target: 5'- uGCCCaUGCcCGGGGCGgUGCUGuuUUa -3' miRNA: 3'- -UGGG-ACGcGUCCCGCgACGACcuAAa -5' |
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| 2807 | 3' | -60.3 | NC_001491.2 | + | 147480 | 0.68 | 0.531519 |
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Target: 5'- cACCCUGCGCuuGGGgGCcGgaGGGc-- -3' miRNA: 3'- -UGGGACGCGu-CCCgCGaCgaCCUaaa -5' |
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| 2807 | 3' | -60.3 | NC_001491.2 | + | 114499 | 0.69 | 0.492642 |
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Target: 5'- gUUCUGC-CGGguGGCGCUGCUGGAg-- -3' miRNA: 3'- uGGGACGcGUC--CCGCGACGACCUaaa -5' |
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| 2807 | 3' | -60.3 | NC_001491.2 | + | 134862 | 0.7 | 0.428026 |
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Target: 5'- cCCCUGCGauagAGGGgGUUGCccuaUGGAUUUg -3' miRNA: 3'- uGGGACGCg---UCCCgCGACG----ACCUAAA- -5' |
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| 2807 | 3' | -60.3 | NC_001491.2 | + | 121773 | 0.77 | 0.14947 |
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Target: 5'- cGCCCUGCGCAGGGUGC-GCgaguccucggucacgUGGAUc- -3' miRNA: 3'- -UGGGACGCGUCCCGCGaCG---------------ACCUAaa -5' |
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| 2807 | 3' | -60.3 | NC_001491.2 | + | 141438 | 1.04 | 0.001829 |
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Target: 5'- cACCCUGCGCAGGGCGCUGCUGGAUUUu -3' miRNA: 3'- -UGGGACGCGUCCCGCGACGACCUAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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