Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2807 | 5' | -57.3 | NC_001491.2 | + | 114761 | 0.69 | 0.711724 |
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Target: 5'- ---aGCGCACcCGCGuuCCCCugGCUCc -3' miRNA: 3'- caugUGCGUGuGCGC--GGGGugUGAGc -5' |
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| 2807 | 5' | -57.3 | NC_001491.2 | + | 60835 | 0.69 | 0.691828 |
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Target: 5'- cGUACAUGCGCcUGCGCUgcgaagcauCCGCAgUCGc -3' miRNA: 3'- -CAUGUGCGUGuGCGCGG---------GGUGUgAGC- -5' |
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| 2807 | 5' | -57.3 | NC_001491.2 | + | 143066 | 0.71 | 0.610957 |
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Target: 5'- aUACACaCACGCGCcgGCCCCACcccaUCGa -3' miRNA: 3'- cAUGUGcGUGUGCG--CGGGGUGug--AGC- -5' |
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| 2807 | 5' | -57.3 | NC_001491.2 | + | 23265 | 0.71 | 0.600838 |
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Target: 5'- -aGCGCGCaacggGCGCGCGCCgCCugGCg-- -3' miRNA: 3'- caUGUGCG-----UGUGCGCGG-GGugUGagc -5' |
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| 2807 | 5' | -57.3 | NC_001491.2 | + | 125547 | 0.72 | 0.511561 |
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Target: 5'- -gGCgGCGCuCGCGCGCuCCCGCgcccGCUCGg -3' miRNA: 3'- caUG-UGCGuGUGCGCG-GGGUG----UGAGC- -5' |
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| 2807 | 5' | -57.3 | NC_001491.2 | + | 123308 | 0.73 | 0.446091 |
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Target: 5'- aGUACGCGCagaagaGCAUGCGgCCgCugACUCGg -3' miRNA: 3'- -CAUGUGCG------UGUGCGCgGG-GugUGAGC- -5' |
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| 2807 | 5' | -57.3 | NC_001491.2 | + | 60890 | 0.74 | 0.41084 |
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Target: 5'- uGUuCGCGCACGCaGCGCCCCAaACUgGc -3' miRNA: 3'- -CAuGUGCGUGUG-CGCGGGGUgUGAgC- -5' |
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| 2807 | 5' | -57.3 | NC_001491.2 | + | 38663 | 0.75 | 0.361338 |
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Target: 5'- uGUGCGCGuCGCGCGCGCCUCccuggugcGCGCUUu -3' miRNA: 3'- -CAUGUGC-GUGUGCGCGGGG--------UGUGAGc -5' |
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| 2807 | 5' | -57.3 | NC_001491.2 | + | 81417 | 0.82 | 0.153082 |
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Target: 5'- -cACGCGUACACGgGCCCgGCGCUCc -3' miRNA: 3'- caUGUGCGUGUGCgCGGGgUGUGAGc -5' |
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| 2807 | 5' | -57.3 | NC_001491.2 | + | 141400 | 1.09 | 0.00231 |
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Target: 5'- gGUACACGCACACGCGCCCCACACUCGa -3' miRNA: 3'- -CAUGUGCGUGUGCGCGGGGUGUGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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