Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2807 | 5' | -57.3 | NC_001491.2 | + | 121556 | 0.66 | 0.839572 |
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Target: 5'- -gGCACGCGCACgGCGUCgaUGCGCUgGc -3' miRNA: 3'- caUGUGCGUGUG-CGCGGg-GUGUGAgC- -5' |
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| 2807 | 5' | -57.3 | NC_001491.2 | + | 123308 | 0.73 | 0.446091 |
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Target: 5'- aGUACGCGCagaagaGCAUGCGgCCgCugACUCGg -3' miRNA: 3'- -CAUGUGCG------UGUGCGCgGG-GugUGAGC- -5' |
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| 2807 | 5' | -57.3 | NC_001491.2 | + | 123373 | 0.68 | 0.741049 |
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Target: 5'- --cCGCGCACACGCGCgCUCGUACaUCu -3' miRNA: 3'- cauGUGCGUGUGCGCG-GGGUGUG-AGc -5' |
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| 2807 | 5' | -57.3 | NC_001491.2 | + | 125547 | 0.72 | 0.511561 |
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Target: 5'- -gGCgGCGCuCGCGCGCuCCCGCgcccGCUCGg -3' miRNA: 3'- caUG-UGCGuGUGCGCG-GGGUG----UGAGC- -5' |
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| 2807 | 5' | -57.3 | NC_001491.2 | + | 137373 | 0.67 | 0.831385 |
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Target: 5'- aGUAaGCGC-CACGCGCCgCCGC-CUg- -3' miRNA: 3'- -CAUgUGCGuGUGCGCGG-GGUGuGAgc -5' |
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| 2807 | 5' | -57.3 | NC_001491.2 | + | 140186 | 0.67 | 0.805787 |
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Target: 5'- -cGCgGCGCACGCGgucuCGCUgCCGCGCUCc -3' miRNA: 3'- caUG-UGCGUGUGC----GCGG-GGUGUGAGc -5' |
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| 2807 | 5' | -57.3 | NC_001491.2 | + | 141400 | 1.09 | 0.00231 |
|
Target: 5'- gGUACACGCACACGCGCCCCACACUCGa -3' miRNA: 3'- -CAUGUGCGUGUGCGCGGGGUGUGAGC- -5' |
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| 2807 | 5' | -57.3 | NC_001491.2 | + | 141651 | 0.68 | 0.769532 |
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Target: 5'- cGUGCGCGUGCcCGCGaggcuCCCCgGCgGCUCGg -3' miRNA: 3'- -CAUGUGCGUGuGCGC-----GGGG-UG-UGAGC- -5' |
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| 2807 | 5' | -57.3 | NC_001491.2 | + | 143066 | 0.71 | 0.610957 |
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Target: 5'- aUACACaCACGCGCcgGCCCCACcccaUCGa -3' miRNA: 3'- cAUGUGcGUGUGCG--CGGGGUGug--AGC- -5' |
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| 2807 | 5' | -57.3 | NC_001491.2 | + | 146571 | 0.67 | 0.787935 |
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Target: 5'- -aGCACGCAgGCGU-CCCCGaGCUCc -3' miRNA: 3'- caUGUGCGUgUGCGcGGGGUgUGAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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