Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28070 | 5' | -59.9 | NC_005887.1 | + | 34945 | 0.67 | 0.332687 |
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Target: 5'- -cGCCGGcuUCGAaCUCGGCG-GGCGu- -3' miRNA: 3'- uaCGGCC--AGCUcGAGCUGCuCCGCua -5' |
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| 28070 | 5' | -59.9 | NC_005887.1 | + | 36983 | 0.7 | 0.204842 |
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Target: 5'- -cGCgGGUCGAGCUCG-Cc-GGCGAg -3' miRNA: 3'- uaCGgCCAGCUCGAGCuGcuCCGCUa -5' |
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| 28070 | 5' | -59.9 | NC_005887.1 | + | 40418 | 0.66 | 0.373938 |
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Target: 5'- cGUGCCGGUCGAaCUCGAgcUGAucaaaucGGCGc- -3' miRNA: 3'- -UACGGCCAGCUcGAGCU--GCU-------CCGCua -5' |
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| 28070 | 5' | -59.9 | NC_005887.1 | + | 40467 | 0.75 | 0.086741 |
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Target: 5'- aAUGCCGGcgucgaCGAGCUCGAuuaCGAGGCGu- -3' miRNA: 3'- -UACGGCCa-----GCUCGAGCU---GCUCCGCua -5' |
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| 28070 | 5' | -59.9 | NC_005887.1 | + | 40723 | 0.67 | 0.340007 |
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Target: 5'- cGUGCCGuccUCGAGCUCGAaUGAcagguagugcguuGGCGGUu -3' miRNA: 3'- -UACGGCc--AGCUCGAGCU-GCU-------------CCGCUA- -5' |
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| 28070 | 5' | -59.9 | NC_005887.1 | + | 40789 | 0.69 | 0.227659 |
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Target: 5'- -cGgCGGUCGAgGUUCGugGcGGCGAc -3' miRNA: 3'- uaCgGCCAGCU-CGAGCugCuCCGCUa -5' |
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| 28070 | 5' | -59.9 | NC_005887.1 | + | 41044 | 0.73 | 0.121807 |
|
Target: 5'- gGUGCaGGUCGAGCUCGA-GAacauGGCGAa -3' miRNA: 3'- -UACGgCCAGCUCGAGCUgCU----CCGCUa -5' |
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| 28070 | 5' | -59.9 | NC_005887.1 | + | 41657 | 0.66 | 0.383663 |
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Target: 5'- -cGUCGG-CGAGCagcaUCGACucGGCGAg -3' miRNA: 3'- uaCGGCCaGCUCG----AGCUGcuCCGCUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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