Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28070 | 5' | -59.9 | NC_005887.1 | + | 23009 | 0.71 | 0.179131 |
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Target: 5'- -cGCCGGUCGc-UUCuGCGAGGCGGUg -3' miRNA: 3'- uaCGGCCAGCucGAGcUGCUCCGCUA- -5' |
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| 28070 | 5' | -59.9 | NC_005887.1 | + | 7731 | 0.71 | 0.160665 |
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Target: 5'- cAUGCCGGguaCGAGCgcgaaaaagGGCGAGGUGAUc -3' miRNA: 3'- -UACGGCCa--GCUCGag-------CUGCUCCGCUA- -5' |
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| 28070 | 5' | -59.9 | NC_005887.1 | + | 5371 | 0.71 | 0.160665 |
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Target: 5'- -cGCCGG-CGAGCUCGACccGcGCGAc -3' miRNA: 3'- uaCGGCCaGCUCGAGCUGcuC-CGCUa -5' |
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| 28070 | 5' | -59.9 | NC_005887.1 | + | 41044 | 0.73 | 0.121807 |
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Target: 5'- gGUGCaGGUCGAGCUCGA-GAacauGGCGAa -3' miRNA: 3'- -UACGgCCAGCUCGAGCUgCU----CCGCUa -5' |
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| 28070 | 5' | -59.9 | NC_005887.1 | + | 40467 | 0.75 | 0.086741 |
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Target: 5'- aAUGCCGGcgucgaCGAGCUCGAuuaCGAGGCGu- -3' miRNA: 3'- -UACGGCCa-----GCUCGAGCU---GCUCCGCua -5' |
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| 28070 | 5' | -59.9 | NC_005887.1 | + | 26686 | 0.75 | 0.084297 |
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Target: 5'- -aGCUGGUCGAGCUCGGCGcgcaucuGCGAc -3' miRNA: 3'- uaCGGCCAGCUCGAGCUGCuc-----CGCUa -5' |
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| 28070 | 5' | -59.9 | NC_005887.1 | + | 19503 | 0.8 | 0.035281 |
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Target: 5'- gAUGCCGG-CGAGCaUCGGCGcAGGCGAc -3' miRNA: 3'- -UACGGCCaGCUCG-AGCUGC-UCCGCUa -5' |
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| 28070 | 5' | -59.9 | NC_005887.1 | + | 11062 | 1.03 | 0.000616 |
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Target: 5'- gAUGCCGGUCGAGCUCGACGAGGCGAUc -3' miRNA: 3'- -UACGGCCAGCUCGAGCUGCUCCGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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