Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28076 | 3' | -55.4 | NC_005887.1 | + | 7603 | 0.66 | 0.641631 |
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Target: 5'- ----aGCGGCGCcgGUUgAUCgaUGGGCGGc -3' miRNA: 3'- ggucaCGCCGCG--CAAgUAG--ACCUGCC- -5' |
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| 28076 | 3' | -55.4 | NC_005887.1 | + | 26406 | 0.67 | 0.619563 |
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Target: 5'- --cGUGCGGCGCGUggccggcgCAUCgauuccGGcccgcGCGGa -3' miRNA: 3'- gguCACGCCGCGCAa-------GUAGa-----CC-----UGCC- -5' |
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| 28076 | 3' | -55.4 | NC_005887.1 | + | 2844 | 0.68 | 0.564745 |
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Target: 5'- -uGGUGCGGCGUgaacuuccaGUUCGUCagccUGGccaGCGGc -3' miRNA: 3'- ggUCACGCCGCG---------CAAGUAG----ACC---UGCC- -5' |
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| 28076 | 3' | -55.4 | NC_005887.1 | + | 8757 | 0.7 | 0.460029 |
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Target: 5'- aCAGUcGUGGCuccugcCGUUCAUCgucgcGGACGGc -3' miRNA: 3'- gGUCA-CGCCGc-----GCAAGUAGa----CCUGCC- -5' |
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| 28076 | 3' | -55.4 | NC_005887.1 | + | 41937 | 0.7 | 0.4403 |
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Target: 5'- aCAGcggGCGGCGCGgcgCGUCUucGCGGc -3' miRNA: 3'- gGUCa--CGCCGCGCaa-GUAGAccUGCC- -5' |
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| 28076 | 3' | -55.4 | NC_005887.1 | + | 20494 | 0.72 | 0.338668 |
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Target: 5'- cUCAGgGCGGCGUGUuugacaccguuuucUCAaauagCUGGAUGGg -3' miRNA: 3'- -GGUCaCGCCGCGCA--------------AGUa----GACCUGCC- -5' |
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| 28076 | 3' | -55.4 | NC_005887.1 | + | 19052 | 0.73 | 0.279792 |
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Target: 5'- gCAGUGCGGCGuUGUUCuUC-GGcGCGGg -3' miRNA: 3'- gGUCACGCCGC-GCAAGuAGaCC-UGCC- -5' |
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| 28076 | 3' | -55.4 | NC_005887.1 | + | 9146 | 1.13 | 0.000447 |
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Target: 5'- gCCAGUGCGGCGCGUUCAUCUGGACGGg -3' miRNA: 3'- -GGUCACGCCGCGCAAGUAGACCUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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