Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28079 | 5' | -55.1 | NC_005887.1 | + | 6295 | 0.66 | 0.587711 |
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Target: 5'- gCGUGCGCGAGCgcgucgaggccuUGCGccucgccGGCGCcGAGGUg -3' miRNA: 3'- -GUACGCGUUCG------------GCGC-------UCGCGaCUUUA- -5' |
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| 28079 | 5' | -55.1 | NC_005887.1 | + | 30629 | 0.66 | 0.577463 |
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Target: 5'- uCGUGCGC--GCCGUGcGCGCggcGAAGc -3' miRNA: 3'- -GUACGCGuuCGGCGCuCGCGa--CUUUa -5' |
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| 28079 | 5' | -55.1 | NC_005887.1 | + | 39346 | 0.67 | 0.543629 |
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Target: 5'- --gGCGCuGGCCGCaGGCGCguUGAc-- -3' miRNA: 3'- guaCGCGuUCGGCGcUCGCG--ACUuua -5' |
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| 28079 | 5' | -55.1 | NC_005887.1 | + | 37690 | 0.67 | 0.543629 |
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Target: 5'- -cUGCGCAgcGGuCUGCGcGuCGCUGAGAUc -3' miRNA: 3'- guACGCGU--UC-GGCGCuC-GCGACUUUA- -5' |
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| 28079 | 5' | -55.1 | NC_005887.1 | + | 23960 | 0.67 | 0.543629 |
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Target: 5'- --gGCGUAGGCCaucggguucGCGAGCGCcGGc-- -3' miRNA: 3'- guaCGCGUUCGG---------CGCUCGCGaCUuua -5' |
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| 28079 | 5' | -55.1 | NC_005887.1 | + | 24615 | 0.66 | 0.611738 |
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Target: 5'- --cGCGC-GGCCGUgccGAGCGUcuUGAAGa -3' miRNA: 3'- guaCGCGuUCGGCG---CUCGCG--ACUUUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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