Results 61 - 64 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
28084 | 3' | -60.1 | NC_005887.1 | + | 798 | 0.66 | 0.413779 |
Target: 5'- gCG-CGAGCUCGCgAUCGCgaucaaCAGCCa-- -3' miRNA: 3'- -GCaGCUCGAGCG-UGGCGa-----GUCGGgcu -5' |
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28084 | 3' | -60.1 | NC_005887.1 | + | 20657 | 0.66 | 0.423053 |
Target: 5'- --aCGAgacGCUCGCACCGUuugCAGCugcccCCGAc -3' miRNA: 3'- gcaGCU---CGAGCGUGGCGa--GUCG-----GGCU- -5' |
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28084 | 3' | -60.1 | NC_005887.1 | + | 38623 | 0.66 | 0.412858 |
Target: 5'- cCGUCcAGCUUGCGCggaucgcggucggCGCgCGGCUCGAc -3' miRNA: 3'- -GCAGcUCGAGCGUG-------------GCGaGUCGGGCU- -5' |
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28084 | 3' | -60.1 | NC_005887.1 | + | 1489 | 0.66 | 0.413779 |
Target: 5'- gCGUgCGGcGCUCGCGCgGCgCGGCCg-- -3' miRNA: 3'- -GCA-GCU-CGAGCGUGgCGaGUCGGgcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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