Results 61 - 64 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28084 | 3' | -60.1 | NC_005887.1 | + | 40356 | 0.73 | 0.128884 |
Target: 5'- cCGUCGAGCgcggcCGUGCCGCUCGucgcGCgCGAg -3' miRNA: 3'- -GCAGCUCGa----GCGUGGCGAGU----CGgGCU- -5' |
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28084 | 3' | -60.1 | NC_005887.1 | + | 40714 | 0.69 | 0.243887 |
Target: 5'- -uUCGAcGCUCGUGCCGUccUCgAGCUCGAa -3' miRNA: 3'- gcAGCU-CGAGCGUGGCG--AG-UCGGGCU- -5' |
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28084 | 3' | -60.1 | NC_005887.1 | + | 41226 | 0.66 | 0.386724 |
Target: 5'- cCGUgacCGAGCUcgacaagcacaCGCACCuGCUCGGCgUCGGc -3' miRNA: 3'- -GCA---GCUCGA-----------GCGUGG-CGAGUCG-GGCU- -5' |
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28084 | 3' | -60.1 | NC_005887.1 | + | 41564 | 0.68 | 0.281513 |
Target: 5'- uCG-CGAGCUCGCGCuCGUUuuuccacugauacgCGuGCCCGAu -3' miRNA: 3'- -GCaGCUCGAGCGUG-GCGA--------------GU-CGGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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