Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28086 | 5' | -54.3 | NC_005887.1 | + | 40355 | 0.7 | 0.437229 |
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Target: 5'- -gCCGUCGAGCGCGgccgugccgcucgUCGCGcGCGAGg -3' miRNA: 3'- caGGUAGCUCGUGU-------------AGUGCcCGCUUg -5' |
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| 28086 | 5' | -54.3 | NC_005887.1 | + | 36964 | 0.71 | 0.408858 |
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Target: 5'- cUCCGUCGucucGGCGUcgCGCGGGuCGAGCu -3' miRNA: 3'- cAGGUAGC----UCGUGuaGUGCCC-GCUUG- -5' |
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| 28086 | 5' | -54.3 | NC_005887.1 | + | 11837 | 0.72 | 0.320356 |
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Target: 5'- uGUUC-UCGAGCAg--CACGGGCGGAUc -3' miRNA: 3'- -CAGGuAGCUCGUguaGUGCCCGCUUG- -5' |
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| 28086 | 5' | -54.3 | NC_005887.1 | + | 14690 | 0.73 | 0.312338 |
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Target: 5'- -aCgAUCGGGCuCGUCgcgGCGGGCGGGCc -3' miRNA: 3'- caGgUAGCUCGuGUAG---UGCCCGCUUG- -5' |
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| 28086 | 5' | -54.3 | NC_005887.1 | + | 18247 | 0.82 | 0.079301 |
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Target: 5'- -gCCAcgcCGGGCAcCGUCACGGGCGAGCu -3' miRNA: 3'- caGGUa--GCUCGU-GUAGUGCCCGCUUG- -5' |
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| 28086 | 5' | -54.3 | NC_005887.1 | + | 6364 | 1.1 | 0.000715 |
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Target: 5'- uGUCCAUCGAGCACAUCACGGGCGAACg -3' miRNA: 3'- -CAGGUAGCUCGUGUAGUGCCCGCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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