Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28097 | 3' | -49.5 | NC_005887.1 | + | 25260 | 0.71 | 0.701189 |
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Target: 5'- --cGUCGUCGAGguauugCGAGCG-GUCGAg -3' miRNA: 3'- guaCAGCAGCUCga----GCUUGCuUAGCU- -5' |
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| 28097 | 3' | -49.5 | NC_005887.1 | + | 40475 | 0.71 | 0.689827 |
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Target: 5'- --cGUCGaCGAGCUCGAuuACGAggCGu -3' miRNA: 3'- guaCAGCaGCUCGAGCU--UGCUuaGCu -5' |
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| 28097 | 3' | -49.5 | NC_005887.1 | + | 11062 | 0.73 | 0.620806 |
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Target: 5'- gAUGcCgGUCGAGCUCG-ACGAggCGAu -3' miRNA: 3'- gUACaG-CAGCUCGAGCuUGCUuaGCU- -5' |
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| 28097 | 3' | -49.5 | NC_005887.1 | + | 31835 | 0.73 | 0.574848 |
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Target: 5'- aCAUGUCGUCGGGaCUcCGAuaugcACG-AUCGAu -3' miRNA: 3'- -GUACAGCAGCUC-GA-GCU-----UGCuUAGCU- -5' |
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| 28097 | 3' | -49.5 | NC_005887.1 | + | 31291 | 0.76 | 0.443955 |
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Target: 5'- ---cUCGUCGAGCUCGAcCGGcAUCGGc -3' miRNA: 3'- guacAGCAGCUCGAGCUuGCU-UAGCU- -5' |
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| 28097 | 3' | -49.5 | NC_005887.1 | + | 2048 | 1.1 | 0.003007 |
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Target: 5'- gCAUGUCGUCGAGCUCGAACGAAUCGAg -3' miRNA: 3'- -GUACAGCAGCUCGAGCUUGCUUAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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