Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28099 | 3' | -56.8 | NC_005887.1 | + | 37456 | 0.67 | 0.528636 |
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Target: 5'- cCUCGcgCCGgcCCUCGGCgaccauCGUC-GCGa -3' miRNA: 3'- -GAGCuaGGCaaGGAGCCG------GCAGaCGU- -5' |
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| 28099 | 3' | -56.8 | NC_005887.1 | + | 33446 | 0.67 | 0.507503 |
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Target: 5'- -aCGuUCCG-UCCUCGGUCGUCagGUc -3' miRNA: 3'- gaGCuAGGCaAGGAGCCGGCAGa-CGu -5' |
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| 28099 | 3' | -56.8 | NC_005887.1 | + | 36288 | 0.67 | 0.497071 |
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Target: 5'- gCUCGAcUCG-UCCggUUGGCCGaUCUGCGc -3' miRNA: 3'- -GAGCUaGGCaAGG--AGCCGGC-AGACGU- -5' |
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| 28099 | 3' | -56.8 | NC_005887.1 | + | 37335 | 0.68 | 0.427053 |
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Target: 5'- uUCGGUCUGcgCCUCGGCgggCGUgaGCu -3' miRNA: 3'- gAGCUAGGCaaGGAGCCG---GCAgaCGu -5' |
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| 28099 | 3' | -56.8 | NC_005887.1 | + | 38019 | 0.7 | 0.354661 |
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Target: 5'- gUCGAUCaGUUCCUgCGGCaUGUCcgGCAg -3' miRNA: 3'- gAGCUAGgCAAGGA-GCCG-GCAGa-CGU- -5' |
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| 28099 | 3' | -56.8 | NC_005887.1 | + | 1236 | 1.09 | 0.000539 |
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Target: 5'- aCUCGAUCCGUUCCUCGGCCGUCUGCAg -3' miRNA: 3'- -GAGCUAGGCAAGGAGCCGGCAGACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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