Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2810 | 5' | -59.7 | NC_001491.2 | + | 146653 | 0.66 | 0.740865 |
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Target: 5'- gAUCUCGCUGGGguccaguccgGgGACGGcGGAc--- -3' miRNA: 3'- -UGGAGCGACCCa---------CgCUGCC-CCUucau -5' |
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| 2810 | 5' | -59.7 | NC_001491.2 | + | 67017 | 0.67 | 0.672084 |
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Target: 5'- gACCacCGCaacGGGUGUGugguUGGGGAGGUGu -3' miRNA: 3'- -UGGa-GCGa--CCCACGCu---GCCCCUUCAU- -5' |
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| 2810 | 5' | -59.7 | NC_001491.2 | + | 144903 | 0.68 | 0.621832 |
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Target: 5'- aGCCggGCUGGGUGC--CGGGGAc--- -3' miRNA: 3'- -UGGagCGACCCACGcuGCCCCUucau -5' |
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| 2810 | 5' | -59.7 | NC_001491.2 | + | 145403 | 0.68 | 0.611774 |
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Target: 5'- aGCCggaGC-GGGUGCGcCGGGGGAa-- -3' miRNA: 3'- -UGGag-CGaCCCACGCuGCCCCUUcau -5' |
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| 2810 | 5' | -59.7 | NC_001491.2 | + | 147294 | 0.71 | 0.457022 |
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Target: 5'- cGCCUCcugGCUGaGGUGgGcCGGGGAGGc- -3' miRNA: 3'- -UGGAG---CGAC-CCACgCuGCCCCUUCau -5' |
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| 2810 | 5' | -59.7 | NC_001491.2 | + | 148790 | 0.71 | 0.421531 |
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Target: 5'- gACC-CGCgGGGcucgGCGGCGGGGAgccucGGUGa -3' miRNA: 3'- -UGGaGCGaCCCa---CGCUGCCCCU-----UCAU- -5' |
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| 2810 | 5' | -59.7 | NC_001491.2 | + | 138279 | 1.06 | 0.001896 |
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Target: 5'- cACCUCGCUGGGUGCGACGGGGAAGUAg -3' miRNA: 3'- -UGGAGCGACCCACGCUGCCCCUUCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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