Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28101 | 5' | -53 | NC_005887.1 | + | 7975 | 0.66 | 0.775464 |
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Target: 5'- -gCCcgG-CCGCCUcUGaUACGCGgggCGCu -3' miRNA: 3'- caGGuaCaGGCGGA-AC-AUGCGUa--GCG- -5' |
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| 28101 | 5' | -53 | NC_005887.1 | + | 32771 | 0.67 | 0.721758 |
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Target: 5'- -gCCAUGcagCCGgCUcGUGCGCAggaucggCGCg -3' miRNA: 3'- caGGUACa--GGCgGAaCAUGCGUa------GCG- -5' |
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| 28101 | 5' | -53 | NC_005887.1 | + | 40506 | 0.67 | 0.721758 |
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Target: 5'- gGUCCGaGUCCGgCgcGUuCGUGUCGCc -3' miRNA: 3'- -CAGGUaCAGGCgGaaCAuGCGUAGCG- -5' |
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| 28101 | 5' | -53 | NC_005887.1 | + | 31169 | 0.67 | 0.710679 |
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Target: 5'- -aUCGUGUCgCGCCacUUGUGCucggGCAUgCGCg -3' miRNA: 3'- caGGUACAG-GCGG--AACAUG----CGUA-GCG- -5' |
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| 28101 | 5' | -53 | NC_005887.1 | + | 33462 | 0.67 | 0.699518 |
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Target: 5'- cGUCag-GUCCGCgagcgcGUACGgCGUCGCg -3' miRNA: 3'- -CAGguaCAGGCGgaa---CAUGC-GUAGCG- -5' |
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| 28101 | 5' | -53 | NC_005887.1 | + | 29289 | 0.73 | 0.359766 |
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Target: 5'- uUCCAcgGaUCgGCCUUGUACGCGgcgaCGCu -3' miRNA: 3'- cAGGUa-C-AGgCGGAACAUGCGUa---GCG- -5' |
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| 28101 | 5' | -53 | NC_005887.1 | + | 25786 | 0.73 | 0.342372 |
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Target: 5'- aGUCCAg--CUGCCUUGgcugcACGCAUCaGCa -3' miRNA: 3'- -CAGGUacaGGCGGAACa----UGCGUAG-CG- -5' |
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| 28101 | 5' | -53 | NC_005887.1 | + | 39754 | 0.76 | 0.250935 |
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Target: 5'- uGUUUuUGUCCGCCUUGUcCGCGUacCGCu -3' miRNA: 3'- -CAGGuACAGGCGGAACAuGCGUA--GCG- -5' |
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| 28101 | 5' | -53 | NC_005887.1 | + | 313 | 1.13 | 0.000685 |
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Target: 5'- gGUCCAUGUCCGCCUUGUACGCAUCGCg -3' miRNA: 3'- -CAGGUACAGGCGGAACAUGCGUAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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