Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28104 | 5' | -56.2 | NC_005887.1 | + | 14620 | 0.73 | 0.246 |
Target: 5'- gGUCUUCcgCGCGCgacaugccgaUCGAcgCGCCgCCGAc -3' miRNA: 3'- -CAGAAGuaGCGCG----------AGCUa-GCGG-GGCU- -5' |
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28104 | 5' | -56.2 | NC_005887.1 | + | 34463 | 0.73 | 0.239637 |
Target: 5'- cGUCUUCGuUCGCGCg-GcUCGCgCCCGAg -3' miRNA: 3'- -CAGAAGU-AGCGCGagCuAGCG-GGGCU- -5' |
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28104 | 5' | -56.2 | NC_005887.1 | + | 16832 | 0.77 | 0.127811 |
Target: 5'- -cCUUCAUCGCG-UCGAUCGCgCCCa- -3' miRNA: 3'- caGAAGUAGCGCgAGCUAGCG-GGGcu -5' |
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28104 | 5' | -56.2 | NC_005887.1 | + | 1670 | 1.09 | 0.000651 |
Target: 5'- gGUCUUCAUCGCGCUCGAUCGCCCCGAg -3' miRNA: 3'- -CAGAAGUAGCGCGAGCUAGCGGGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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