Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2811 | 3' | -62.4 | NC_001491.2 | + | 147774 | 0.66 | 0.643577 |
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Target: 5'- cGCCAGCUCcccgaagcGCGCgCCGUcccggGCCGgGGGg -3' miRNA: 3'- -CGGUCGGG--------UGUG-GGCGca---CGGCaCCC- -5' |
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| 2811 | 3' | -62.4 | NC_001491.2 | + | 114970 | 0.66 | 0.643577 |
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Target: 5'- gGCC-GCCCugACuCCGCGggucgccugcGCCGUGc- -3' miRNA: 3'- -CGGuCGGGugUG-GGCGCa---------CGGCACcc -5' |
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| 2811 | 3' | -62.4 | NC_001491.2 | + | 116732 | 0.66 | 0.614382 |
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Target: 5'- gGCCcucgGGCCCcgacgcaagGCugCCGCGgccgcgGCCGcuccgGGGa -3' miRNA: 3'- -CGG----UCGGG---------UGugGGCGCa-----CGGCa----CCC- -5' |
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| 2811 | 3' | -62.4 | NC_001491.2 | + | 63826 | 0.66 | 0.614382 |
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Target: 5'- uCCAGCUCA-GCCCGCGUGUacaccuccaGGGg -3' miRNA: 3'- cGGUCGGGUgUGGGCGCACGgca------CCC- -5' |
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| 2811 | 3' | -62.4 | NC_001491.2 | + | 116828 | 0.67 | 0.604666 |
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Target: 5'- cCCGGCCCAgGCcgCCGC-UGCCGcGGc -3' miRNA: 3'- cGGUCGGGUgUG--GGCGcACGGCaCCc -5' |
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| 2811 | 3' | -62.4 | NC_001491.2 | + | 34483 | 0.67 | 0.604666 |
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Target: 5'- gGCCAcgcgcGCCCugGCCCGgGacgaacGUCGUcGGGu -3' miRNA: 3'- -CGGU-----CGGGugUGGGCgCa-----CGGCA-CCC- -5' |
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| 2811 | 3' | -62.4 | NC_001491.2 | + | 49648 | 0.79 | 0.11394 |
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Target: 5'- cGCCGGCCU--GCCCGCuccaugucUGCCGUGGGg -3' miRNA: 3'- -CGGUCGGGugUGGGCGc-------ACGGCACCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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