Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2811 | 3' | -62.4 | NC_001491.2 | + | 125944 | 0.69 | 0.482066 |
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Target: 5'- aUCAGCCCuauCACCUcuGgGUGCUG-GGGg -3' miRNA: 3'- cGGUCGGGu--GUGGG--CgCACGGCaCCC- -5' |
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| 2811 | 3' | -62.4 | NC_001491.2 | + | 114769 | 0.69 | 0.4642 |
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Target: 5'- cGCCGGCgagCGCACCCGCGUuccccuggcuCCGaGGGa -3' miRNA: 3'- -CGGUCGg--GUGUGGGCGCAc---------GGCaCCC- -5' |
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| 2811 | 3' | -62.4 | NC_001491.2 | + | 140176 | 0.7 | 0.412799 |
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Target: 5'- -gCGGUCuCGCugCCGCGcuCCGUGGGg -3' miRNA: 3'- cgGUCGG-GUGugGGCGCacGGCACCC- -5' |
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| 2811 | 3' | -62.4 | NC_001491.2 | + | 143882 | 0.71 | 0.364302 |
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Target: 5'- gGCCAGCCCACACUaucgauUGUGauugGCCGUugaugauGGGc -3' miRNA: 3'- -CGGUCGGGUGUGG------GCGCa---CGGCA-------CCC- -5' |
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| 2811 | 3' | -62.4 | NC_001491.2 | + | 1790 | 0.72 | 0.307586 |
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Target: 5'- cGCC-GUCCACGCCCGCG-GCCGc--- -3' miRNA: 3'- -CGGuCGGGUGUGGGCGCaCGGCaccc -5' |
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| 2811 | 3' | -62.4 | NC_001491.2 | + | 140021 | 0.74 | 0.240788 |
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Target: 5'- gGCCAGcCCCAUGCCCgucuuccacucgGCGgacccgcugcgccgcGCCGUGGGg -3' miRNA: 3'- -CGGUC-GGGUGUGGG------------CGCa--------------CGGCACCC- -5' |
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| 2811 | 3' | -62.4 | NC_001491.2 | + | 49648 | 0.79 | 0.11394 |
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Target: 5'- cGCCGGCCU--GCCCGCuccaugucUGCCGUGGGg -3' miRNA: 3'- -CGGUCGGGugUGGGCGc-------ACGGCACCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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